W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States.
ACS Appl Mater Interfaces. 2024 Sep 18;16(37):48955-48968. doi: 10.1021/acsami.4c05588. Epub 2024 Aug 28.
Complex bone diseases such as osteomyelitis, osteosarcoma, and osteoporosis often cause critical-size bone defects that the body cannot self-repair and require an advanced bone graft material to repair. We have fabricated 3D-printed tricalcium phosphate bone scaffolds functionalized with garlic extract (GE). GE was encapsulated in a nanoemulsion (GE-NE) to enhance bioavailability and stability. GE-NE showed ∼73% drug encapsulation efficiency, with an average particle size of 158 nm and a zeta potential of -14.2 mV. Release of GE-NEs from the scaffold displayed a controlled and biphasic release profile at both acidic and physiological mediums. Results from the osteosarcoma study show that GE-NE demonstrated ∼88% reduction in cancer cell growth while exhibiting no cytotoxicity toward bone-forming cells. Interaction for the functionalized scaffold with Gram-positive and Gram-negative showed a substantial reduction in bacteria growth by more than 90% compared to the unfunctionalized scaffold. These findings demonstrate the potential of GE-NEs-treated porous scaffolds to treat bone-related diseases, particularly for non-load bearing applications.
复杂的骨病，如骨髓炎、骨肉瘤和骨质疏松症，常导致临界尺寸的骨缺损，机体无法自我修复，需要先进的骨移植物来修复。我们制备了载大蒜提取物（GE）的 3D 打印磷酸三钙骨支架。GE 被包裹在纳米乳液（GE-NE）中，以提高生物利用度和稳定性。GE-NE 的药物包封效率约为 73%，平均粒径为 158nm，zeta 电位为-14.2mV。支架中 GE-NE 的释放呈现出在酸性和生理介质中均具有控制和两相释放的特征。骨肉瘤研究的结果表明，GE-NE 使癌细胞生长减少了约 88%，而对成骨细胞没有细胞毒性。功能化支架与革兰氏阳性菌和革兰氏阴性菌的相互作用表明，与未功能化支架相比，细菌生长减少了 90%以上。这些发现表明，载有 GE-NE 的多孔支架在治疗骨相关疾病方面具有潜力，特别是对于非承重应用。
