W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington, USA.
J Biomed Mater Res A. 2024 Dec;112(12):2110-2123. doi: 10.1002/jbm.a.37756. Epub 2024 Jun 18.
Increasing bone diseases and anomalies significantly challenge bone regeneration, necessitating the development of innovative implantable devices for effective healing. This study explores the potential of 3D-printed calcium phosphate (CaP) scaffolds functionalized with natural medicine to address this issue. Specifically, quercetin and vitamin D3 (QVD) encapsulated solid lipid nanoparticles (QVD-SLNs) are incorporated into the scaffold to enhance bone regeneration. The melt emulsification method is utilized to achieve high drug encapsulation efficiency (~98%) and controlled biphasic release kinetics. The process-structure-property performance of these systems allows more controlled release while maintaining healthy cell-material interactions. The functionalized scaffolds show ~1.3- and ~-1.6-fold increase in osteoblast cell proliferation and differentiation, respectively, as compared with the control. The treated scaffold demonstrates a reduction in osteoclastic activity as compared with the control. The QVD-SLN-loaded scaffolds show ~4.2-fold in vitro chemopreventive potential against osteosarcoma cells. Bacterial assessment with both Staphylococcus aureus and Pseudomonas aeruginosa shows a significant reduction in bacterial colony growth over the treated scaffold. These findings summarize that the release of QVD-SLNs through a 3D-printed CaP scaffold can treat various bone-related disorders for low or non-load-bearing applications.
日益增多的骨病和畸形对骨再生构成了重大挑战，这就需要开发创新的植入式设备来实现有效的治疗。本研究探索了 3D 打印的磷酸钙（CaP）支架与天然药物功能化相结合的潜力，以解决这一问题。具体而言，将槲皮素和维生素 D3（QVD）包封的固体脂质纳米粒（QVD-SLNs）掺入支架中以增强骨再生。采用熔融乳化法实现了高药物包封效率（约 98%）和控制的双相释放动力学。这些系统的工艺-结构-性能表现允许更受控的释放，同时保持健康的细胞-材料相互作用。与对照相比，功能化支架的成骨细胞增殖和分化分别增加了约 1.3 倍和 1.6 倍。与对照相比，处理后的支架显示出降低的破骨细胞活性。负载 QVD-SLN 的支架在体外对骨肉瘤细胞的化学预防潜力约为 4.2 倍。金黄色葡萄球菌和铜绿假单胞菌的细菌评估表明，经处理的支架上的细菌菌落生长显著减少。这些研究结果表明，通过 3D 打印的 CaP 支架释放 QVD-SLNs 可用于治疗各种与骨骼相关的疾病，适用于低负荷或无负荷的应用。
