人血小板中的磷脂生物合成。溶血血小板活化因子的酰化作用。
Phospholipid biosynthesis in human platelets. The acylation of lyso-platelet-activating factor.
作者信息
McKean M L, Silver M J
出版信息
Biochem J. 1985 Feb 1;225(3):723-9. doi: 10.1042/bj2250723.
Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acyltransferase of human platelets is membrane-bound, has a pH optimum of 7.5, is insensitive to 1 mM-Mg2+, is inhibited by 1 mM-Ca2+, and is stimulated slightly by 1 mM-EDTA. Maximal formation of 1-alkyl-2-acyl-sn-glycero-3-phosphocholine is observed at 150 microM-1-alkyl-sn-glycero-3-phosphocholine and 20 microM unsaturated fatty acyl-CoA. The transfer of unsaturated fatty acyl groups to 1-alkyl-sn-glycero-3-phosphocholine is 3-14 times slower than to 1-acyl-sn-glycero-3-phosphocholine. The CoA esters of linoleate and arachidonate, two unsaturated fatty acyl groups commonly found in platelet phospholipids, are the preferred fatty acyl group donors.
人血小板的酰基辅酶A：1-烷基-sn-甘油-3-磷酸胆碱酰基转移酶是膜结合的，最适pH为7.5，对1 mM Mg2+不敏感，被1 mM Ca2+抑制，并被1 mM EDTA轻微刺激。在150 microM 1-烷基-sn-甘油-3-磷酸胆碱和20 microM不饱和脂肪酰基辅酶A时观察到1-烷基-2-酰基-sn-甘油-3-磷酸胆碱的最大生成。不饱和脂肪酰基转移到1-烷基-sn-甘油-3-磷酸胆碱的速度比对1-酰基-sn-甘油-3-磷酸胆碱慢3至14倍。亚油酸和花生四烯酸的辅酶A酯是血小板磷脂中常见的两种不饱和脂肪酰基，是优选的脂肪酰基供体。
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