Clark P O, Hanahan D J, Pinckard R N
Biochim Biophys Acta. 1980 Feb 21;628(1):69-75. doi: 10.1016/0304-4165(80)90352-9.
Platelet-activating factor secreted by stimulated human neutrophils and monocytes and rabbit neutrophils and basophils was isolated, purified and chemically and physically characterized. All four preparations had identical thin layer chromatographic behavior in a variety of solvent systems, identical solubility behavior in various organic and aqueous solvents and responded comparably to a variety of chemical and physical agents designed to reveal the presence functional groups. These findings indicate that human neutrophil and monocyte, and rabbit neutrophil and basophil derived platelet-activating factor preparations are indistinguishable, and support the conclusion that platelet-activating factor is a neutral, polar lipid molecule whose functional activity is dependent upon the presence of a carboxylic acid ester.
对受刺激的人中性粒细胞和单核细胞以及兔中性粒细胞和嗜碱性粒细胞分泌的血小板激活因子进行了分离、纯化，并对其化学和物理性质进行了表征。在多种溶剂系统中，所有这四种制剂都具有相同的薄层色谱行为，在各种有机溶剂和水性溶剂中具有相同的溶解行为，并且对旨在揭示官能团存在的各种化学和物理试剂反应相当。这些发现表明，人中性粒细胞和单核细胞以及兔中性粒细胞和嗜碱性粒细胞衍生的血小板激活因子制剂无法区分，并支持血小板激活因子是一种中性极性脂质分子的结论，其功能活性取决于羧酸酯的存在。
