State Key Laboratory of Pollution Control and Resource Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Laboratory of Solid Waste Environmental Risk Control, Guangdong Provincial Academy of Environmental Science, Guangzhou 510045, China.
J Hazard Mater. 2024 Nov 5;479:135668. doi: 10.1016/j.jhazmat.2024.135668. Epub 2024 Aug 27.
In this study, MgFeO-loaded biochar (MFBC) was used as a three-dimensional particle electrode to active peroxymonosulfate (EC/MFBC/PMS) for the removal of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). The results demonstrated that, under the conditions of 1.0 mM PMS concentration, 0.4 g/L material dosage, 5 V voltage intensity, and MFBC preparation temperature of 600 °C, the EC/MFBC600/PMS system achieved complete inactivation of E. coli DH5α within 5 min and the intracellular sul1 was reduced by 81.5 % after 30 min of the treatment. Compared to EC and PMS alone treatments, the conjugation transfer frequency of sul1 rapidly declined by 92.9 % within 2 min. The cell membrane, proteins, lipids, as well as intracellular and extracellular ARGs in E. coli DH5α were severely damaged by free radicals in solution and intracellular reactive oxygen species (ROS). Furthermore, up-regulation was observed in genes associated with oxidative stress, SOS response and cell membrane permeability in E. coli DH5α, however, no significant changes were observed in functional genes related to gene conjugation and transfer mechanisms. This study would contribute to the underlying of PMS activation by three-dimensional particle electrode, and provide novel insights into the mechanism of ARB inactivation and ARGs degradation under PMS advanced oxidation treatment.
在这项研究中，负载 MgFeO 的生物炭 (MFBC) 被用作三维颗粒电极来激活过一硫酸盐（EC/MFBC/PMS），以去除抗生素耐药菌 (ARB) 和抗生素耐药基因 (ARGs)。结果表明，在 1.0 mM PMS 浓度、0.4 g/L 材料用量、5 V 电压强度和 600°C 的 MFBC 制备温度条件下，EC/MFBC600/PMS 系统在 5 分钟内完全灭活了 E. coli DH5α，并且在 30 分钟的处理后，细胞内 sul1 减少了 81.5%。与 EC 和 PMS 单独处理相比，sul1 的接合转移频率在 2 分钟内迅速下降了 92.9%。细胞膜、蛋白质、脂质以及 E. coli DH5α 中的胞内和胞外 ARGs 均受到溶液中自由基和胞内活性氧（ROS）的严重破坏。此外，在 E. coli DH5α 中，与氧化应激、SOS 反应和细胞膜通透性相关的基因上调，但与基因接合和转移机制相关的功能基因没有明显变化。本研究将有助于深入了解三维颗粒电极对 PMS 的激活机制，并为 PMS 高级氧化处理下 ARB 失活和 ARGs 降解的机制提供新的见解。
