(Burm. f.) Lindau Extract Inhibits Dengue Virus Infection and Inflammation in the Huh7 Hepatoma Cell Line.

Dengue virus (DENV) infection has emerged as a global health problem, with no specific treatment available presently. (Burm. f.) Lindau extract has been used in traditional medicine for its anti-inflammatory and antiviral properties. We thus hypothesized had a broad-ranged activity to inhibit DENV and the liver inflammation caused by DENV infection. The study showed that treatment using extract during DENV infection (co-infection step) showed the highest efficiency in lowering the viral antigen concentration to 22.87 ± 6.49% at 31.25 μg/mL. In addition, the virus-host cell binding assay demonstrated that treatment greatly inhibited the virus after its binding to Huh7 cells. Moreover, it could remarkably lower the expression of cytokine and chemokine genes, including , , , and , in addition to inflammatory mediator genes. Interestingly, the activation of the NF-κB signaling cascade after extract treatment was dramatically decreased, which could be the underlying mechanism of its anti-inflammatory activity. The HPLC profile showed that gallic acid was the bioactive compound of extract and might be responsible for the antiviral properties of . Taken together, our results revealed the potential of extract to inhibit DENV infection and lower inflammation in infected cells.