Panya Aussara, Pundith Hataichanok, Thongyim Supawadee, Kaewkod Thida, Chitov Thararat, Bovonsombut Sakunnee, Tragoolpua Yingmanee
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Research Center in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Antibiotics (Basel). 2020 Jul 21;9(7):429. doi: 10.3390/antibiotics9070429.
Mastitis caused by bacterial infection has negative impacts on milk quality and animal health, and ultimately causes economic losses to the dairy industry worldwide. Gram-negative bacteria and their component lipopolysaccharide (LPS) can trigger the inflammatory response of endothelial cells (ECs) and subsequently promote EC dysfunction or injury, which is a critical pathogenesis of mastitis-causing sepsis shock. To control the bacterial infection and to minimise the LPS negative effects on ECs, we thus aimed to identify the potential herb extracts that comprised antibacterial activity and protective ability to inhibit LPS-induced cell death. Extracts from seven types of herbs derived from antibacterial screening were investigated for their protective effects on LPS-stimulated bovine endothelial cell line. (Burm. f.) Lindau () extract appeared to be the most effective antiapoptotic extract against LPS stimulation. Treatment of extract in LPS-stimulated cells significantly lowered apoptotic cell death through modulating pro-survival Bcl-2 and pro-apoptotic Bax expression. The investigation of bioactive compounds using solvent fractionation, HPLC, and LC-MS/MS analysis revealed glyceryl 1,3-disterate (CHO), kaempferol 3-O-feruloyl-sophoroside 7-O-glucoside (CHO), and hydroxypthioceranic acid (CHO) as the candidate components. Our findings indicated that extract has great potential to be further developed as an alternative therapeutic agent for mastitis treatment.
由细菌感染引起的乳腺炎会对牛奶质量和动物健康产生负面影响，并最终给全球乳制品行业造成经济损失。革兰氏阴性菌及其成分脂多糖（LPS）可引发内皮细胞（ECs）的炎症反应，随后促进EC功能障碍或损伤，这是导致乳腺炎败血症休克的关键发病机制。为了控制细菌感染并将LPS对ECs的负面影响降至最低，我们旨在鉴定具有抗菌活性和保护能力以抑制LPS诱导的细胞死亡的潜在草药提取物。对七种经抗菌筛选的草药提取物对LPS刺激的牛内皮细胞系的保护作用进行了研究。（Burm. f.）Lindau（）提取物似乎是针对LPS刺激最有效的抗凋亡提取物。在LPS刺激的细胞中用提取物处理可通过调节促生存Bcl-2和促凋亡Bax的表达，显著降低凋亡细胞死亡。使用溶剂分级分离、高效液相色谱（HPLC）和液相色谱-质谱联用/质谱（LC-MS/MS）分析对生物活性化合物进行的研究表明，甘油1,3-二硬脂酸酯（CHO）、山奈酚3-O-阿魏酰槐糖苷7-O-葡萄糖苷（CHO）和羟基硫代神经酸（CHO）为候选成分。我们的研究结果表明，提取物具有很大的潜力可进一步开发成为治疗乳腺炎的替代治疗剂。
