Tan Li, Ma Rong, Katz Adam J, Levi Nicole
Department of Plastic and Reconstructive Surgery, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Antibiotics (Basel). 2024 Aug 17;13(8):778. doi: 10.3390/antibiotics13080778.
The family of ESKAPE pathogens is comprised of , and . Together they are the main contributors of nosocomial infections and are well established for their ability to "escape" antibiotics. Farnesol is an FDA-approved cosmetic and flavoring agent with significant anti-biofilm properties. In a proprietary emulsion, farnesol has been shown to be capable of disrupting , and biofilms. The current work demonstrates that this farnesol emulsion reduces the number of viable bacteria, while also leading to reductions in biomass, of the other three ESKAPE pathogens: and both in vitro and in an ex vivo human skin model. A concentration of 0.5 mg/mL was effective for impeding biofilm development of all three bacteria, while 1 mg/mL for and , or 0.2 mg/mL for was able to kill bacteria in established biofilms. Contrary to antibiotics, no resistance to farnesol was observed for or . The results indicate that farnesol is effective for direct cell killing and also has the ability to induce biofilm detachment from surfaces, as confirmed using Live/Dead image analysis. Our findings confirm that farnesol emulsion is an effective broad-spectrum agent to impede ESKAPE biofilms.
ESKAPE 病原体家族由……组成。它们共同构成了医院感染的主要致病菌，并且以其 “逃避” 抗生素的能力而闻名。法尼醇是一种经美国食品药品监督管理局（FDA）批准的化妆品和调味剂，具有显著的抗生物膜特性。在一种专利乳液中，法尼醇已被证明能够破坏……生物膜。目前的研究表明，这种法尼醇乳液可减少其他三种 ESKAPE 病原体（……）的活菌数量，同时还能在体外和离体人类皮肤模型中减少生物量。0.5 mg/mL 的浓度可有效阻止这三种细菌的生物膜形成，而 1 mg/mL 对……或 0.2 mg/mL 对……能够杀死已形成生物膜中的细菌。与抗生素不同，未观察到……对法尼醇产生耐药性。结果表明，法尼醇对直接杀死细胞有效，并且如通过活/死图像分析所证实的那样，还具有诱导生物膜从表面脱离的能力。我们的研究结果证实，法尼醇乳液是一种有效的广谱制剂，可阻碍 ESKAPE 生物膜的形成。
