Semeshin V F, Baricheva E M, Belyaeva E S, Zhimulev I F
Chromosoma. 1985;91(3-4):210-33. doi: 10.1007/BF00328218.
Data are presented of electron microscopic (EM) analysis of consecutive developmental stages of Drosophila melanogaster complex puffs, formed as a result of simultaneous decondensation of several bands. EM mapping principles proposed by us permitted more exact determination of the banding patterns of 19 regions in which 31 puffs develop. It is shown that 20 of them develop as a result of synchronous decondensation of two bands, 7 of three and 4 of one band. Three cases of two-band puff formation when one or both bands undergo partial decondensation are described. In the 50CF, 62CE, 63F and 71CF regions puffing zones are located closely adjacent to each other but the decondensation of separate band groups occurs at different puff stages (PS). These data are interpreted as activation of independently regulated DNA sequences. The decondensation of two or three adjacent bands during formation of the majority of the puffs occurs simultaneously in the very first stages of their development. It demonstrates synchronous activation of the material of several bands presumably affected by a common inductor. Bands adjacent to puffing centres also lose their clarity as the puff develops, probably due to "passive" decondensation connected with puff growth. The morphological data obtained suggest a complex genetic organisation of many puffs.
本文呈现了对果蝇复杂胀泡连续发育阶段的电子显微镜（EM）分析数据，这些胀泡是由几条带同时解聚形成的。我们提出的EM图谱绘制原则使得能够更精确地确定19个区域的带型，其中有31个胀泡发育。结果表明，其中20个胀泡是由两条带同步解聚形成的，7个由三条带形成，4个由一条带形成。描述了两带胀泡形成的三种情况，即其中一条或两条带发生部分解聚。在50CF、62CE、63F和71CF区域，胀泡区彼此紧邻，但不同带组的解聚发生在不同的胀泡阶段（PS）。这些数据被解释为独立调控的DNA序列的激活。大多数胀泡形成过程中两条或三条相邻带的解聚在其发育的最初阶段同时发生。这表明可能受共同诱导物影响的几条带的物质同步激活。随着胀泡的发育，与胀泡中心相邻的带也会失去清晰度，这可能是由于与胀泡生长相关的“被动”解聚。所获得的形态学数据表明许多胀泡具有复杂的遗传组织。
