Li Jie, Zheng Haonan, Liu Jiahui, Ding Jie, Guo Qingqi, Zhang Na
College of Life Science, Northeast Forestry University, Harbin 150040, China.
College of Food Engineering, Harbin University of Commerce, Harbin 150028, China.
Antioxidants (Basel). 2024 Dec 25;14(1):14. doi: 10.3390/antiox14010014.
Constipation is a prevalent global health issue that greatly affects human well-being. However, many existing treatments are associated with side effects, necessitating the development of alternative approaches. In this study, a balanced fatty acid red pine seed direct-drinking oil (SFA:MUFA:PUFA = 1.14:1.08:1, n - 6:n - 3 = 4.17:1) was formulated using red pine seed oil as the base oil, blended with coconut oil, rice bran oil, and camellia oil. The study investigated the effects and mechanisms of this red pine seed direct-drinking oil in alleviating constipation in mice. Results showed that, compared to normal mice, constipated mice exhibited symptoms of dry stools, difficulty defecating, abnormal neurotransmitter levels, oxidative stress, and colonic tissue damage. Additionally, the protein expression levels of occludin and claudin-1 were reduced by 86.11% and 25.00%, respectively ( < 0.05), while mRNA expression levels decreased by 70.80% and 59.00% ( < 0.05). Red pine seed direct-drinking oil intake improved defecation, reduced serum levels of vasoactive intestinal peptide (VIP), endothelin-1 (ET-1), and nitric oxide (NO), and increased substance P (SP) levels. Furthermore, it also significantly elevated serum levels of superoxide dismutase (SOD) and catalase (CAT) ( < 0.05), alleviated colonic tissue damage, and upregulated the protein and mRNA expression levels of occludin and claudin-1 ( < 0.05). These findings suggest that red pine seed direct-drinking oil alleviates constipation in mice by enhancing intestinal motility, regulating serum neurotransmitters, mitigating oxidative stress, repairing intestinal barrier damage, and increasing tight junction protein expression. This study represents the first use of red pine seed direct-drinking oil to alleviate constipation in mice, providing a novel approach to improving symptoms in individuals with constipation.
便秘是一个普遍存在的全球性健康问题，对人类福祉有很大影响。然而，许多现有治疗方法都伴有副作用，因此需要开发替代方法。在本研究中，以红松籽油为基础油，与椰子油、米糠油和山茶油混合，配制出一种脂肪酸平衡的红松籽直饮油（饱和脂肪酸：单不饱和脂肪酸：多不饱和脂肪酸 = 1.14:1.08:1，n - 6:n - 3 = 4.17:1）。该研究调查了这种红松籽直饮油在缓解小鼠便秘方面的作用及机制。结果显示，与正常小鼠相比，便秘小鼠表现出大便干结、排便困难、神经递质水平异常、氧化应激以及结肠组织损伤等症状。此外，闭合蛋白和紧密连接蛋白 - 1的蛋白表达水平分别降低了86.11%和25.00%（P < 0.05），而mRNA表达水平分别降低了70.80%和59.00%（P < 0.05）。摄入红松籽直饮油改善了排便情况，降低了血清中血管活性肠肽（VIP）、内皮素 - 1（ET - 1）和一氧化氮（NO）的水平，并提高了P物质（SP）的水平。此外，它还显著提高了血清中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的水平（P < 0.05），减轻了结肠组织损伤，并上调了闭合蛋白和紧密连接蛋白 - 1的蛋白及mRNA表达水平（P < 0.05）。这些发现表明，红松籽直饮油通过增强肠道蠕动、调节血清神经递质、减轻氧化应激、修复肠道屏障损伤以及增加紧密连接蛋白表达来缓解小鼠便秘。本研究首次使用红松籽直饮油缓解小鼠便秘，为改善便秘患者的症状提供了一种新方法。
