Cásedas Guillermo, Moliner Cristina, Abad-Longas Alba, Núñez Sonia, Gómez-Rincón Carlota, Maggi Filippo, López Víctor
Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Zaragoza, Spain.
Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50009 Zaragoza, Spain.
Foods. 2024 Aug 17;13(16):2577. doi: 10.3390/foods13162577.
Black mulberry ( L.) is a common edible fruit from the Moraceae family with a wide variety of nutritional and medicinal applications, mainly due to its antioxidant and anti-inflammatory properties. The purpose of this work was to investigate the cytoprotective and neuroprotective capacity of a hydrophilic black mulberry solvent-free extract rich in polyphenols, including the antioxidant, antiradical, and enzymatic mechanisms that would explain these effects. Its neuroprotective potential was evaluated in vitro using the Neuro-2a cell line and in vivo through the organism model. Neuro-2a cells were treated at different concentrations of the extract (25-500 µg/mL) and hydrogen peroxide (300 µM) as an oxidant agent, simultaneously. From these treatments, redox status (intracellular ROS production) and cellular activity (MTT) were also quantified in Neuro-2a. Regarding the assay, the protection of the extract against β-amyloid toxicity was measured against the CL4176 strain, which is a model of Alzheimer disease. As a complementary neuroprotective assay, its potential to inhibit the monoamine oxidase A (MAO-A) enzyme was measured. In addition, an bioassay was performed for preliminary toxicity screening. And its antioxidant properties were evaluated by means of the FRAP assay. The results confirm its neuroprotective potential and its ability to scavenge free radicals and decrease ROS production, also acting as a moderate MAO-A inhibitor. Moreover, the polyphenolic extract alleviates the toxicity induced by β-amyloid accumulation in . Concluding, can be considered a functional food with bioactive compounds that may prevent the onset of neurodegenerative diseases.
黑桑（L.）是桑科一种常见的可食用水果，具有广泛的营养和药用价值，主要归因于其抗氧化和抗炎特性。本研究的目的是调查富含多酚的亲水性黑桑无溶剂提取物的细胞保护和神经保护能力，包括解释这些作用的抗氧化、抗自由基和酶促机制。使用Neuro-2a细胞系在体外评估其神经保护潜力，并通过生物体模型在体内进行评估。将Neuro-2a细胞同时用不同浓度的提取物（25 - 500 µg/mL）和作为氧化剂的过氧化氢（300 µM）处理。从这些处理中，还对Neuro-2a中的氧化还原状态（细胞内活性氧产生）和细胞活性（MTT）进行了定量。关于该实验，针对阿尔茨海默病模型CL4176菌株测量提取物对β-淀粉样蛋白毒性的保护作用。作为补充的神经保护实验，测量其抑制单胺氧化酶A（MAO-A）的潜力。此外，进行了生物测定以进行初步毒性筛选。并通过FRAP实验评估其抗氧化性能。结果证实了其神经保护潜力、清除自由基和减少活性氧产生的能力，还可作为中度MAO-A抑制剂。此外，多酚提取物减轻了β-淀粉样蛋白积累在体内诱导的毒性。总之，黑桑可被认为是一种具有生物活性化合物的功能性食品，可能预防神经退行性疾病的发生。
