Ernst P B, Petit A, Befus A D, Clark D A, Rosenthal K L, Ishizaka T, Bienenstock J
Eur J Immunol. 1985 Mar;15(3):216-21. doi: 10.1002/eji.1830150303.
Highly enriched preparations of intraepithelial lymphocytes (IEL) containing a large subpopulation of granulated cells were isolated from the murine small intestinal mucosa. We cultured IEL in media containing interleukin 2 (growth media conditioned with 20% concanavalin A supernatant; Con A CM) or mast cell growth factor(s) (growth media conditioned with 40% WEHI-3 supernatant; WEHI CM) and compared the physical and functional properties of the cultured cells to freshly isolated IEL. IEL cultured in Con A CM developed enhanced cytotoxicity against YAC-1, compared to freshly isolated IEL, and spontaneous cytotoxicity for P815 targets. Most of these cultured cells were Thy-1+ Lyt-1- Lyt-2+, and contained cytoplasmic granules similar to those seen in electron photomicrographs of other cytotoxic cell populations. IEL cultured in WEHI CM gave rise to cells that morphologically resembled mast cells. Unlike freshly isolated IEL, the cells stained metachromatically, contained 200-450 ng of histamine/10(6) cells and expressed high-affinity receptors for IgE. Our data clearly show that, although IEL do not themselves have physical characteristics of mast cells, they do contain mast cell precursors. In addition, IEL grown in the presence of T cell growth factors give rise to an activated cytotoxic cell population which is mostly granulated and Thy-1+ Lyt-1- Lyt-2+.
从小鼠小肠黏膜中分离出高度富集的上皮内淋巴细胞（IEL）制剂，其中含有大量颗粒细胞亚群。我们将IEL在含有白细胞介素2的培养基（用20%伴刀豆球蛋白A上清液处理的生长培养基；伴刀豆球蛋白A条件培养基）或肥大细胞生长因子的培养基（用40% WEHI-3上清液处理的生长培养基；WEHI条件培养基）中培养，并将培养细胞的物理和功能特性与新鲜分离的IEL进行比较。与新鲜分离的IEL相比，在伴刀豆球蛋白A条件培养基中培养的IEL对YAC-1的细胞毒性增强，对P815靶标的自发细胞毒性增强。这些培养细胞大多为Thy-1+ Lyt-1- Lyt-2+，并含有与其他细胞毒性细胞群体电子显微照片中所见相似的细胞质颗粒。在WEHI条件培养基中培养的IEL产生了形态上类似于肥大细胞的细胞。与新鲜分离的IEL不同，这些细胞呈异染性染色，每10(6)个细胞含有200 - 450 ng组胺，并表达高亲和力IgE受体。我们的数据清楚地表明，尽管IEL本身不具有肥大细胞的物理特征，但它们确实含有肥大细胞前体。此外，在T细胞生长因子存在下生长的IEL产生了一个活化的细胞毒性细胞群体，该群体大多为颗粒状且为Thy-1+ Lyt-1- Lyt-2+。
