van der Heijden F L
Immunology. 1986 Nov;59(3):397-9.
Lymphocyte subpopulations in the intestinal mucosa of the rat were quantified in situ and compared with data obtained by other authors using enzymic or mechanical methods (Selby et al., 1984; Gibson et al., 1985) in order to assess any selective loss of cell types or contamination with lamina propria lymphocytes after these enzymic or mechanical isolation procedures. Intraepithelial lymphocytes (IEL) were predominantly of the suppressor/cytotoxic phenotype (MRC OX-8) and nearly all cells bore the pan T marker W3/13. About 10% of the IEL phenotypically belonged to the T-helper (W3/25) lineage. MRC OX-19, the rat equivalent of the mouse Lyt-1 antigen, was present on about 15% of the IEL. The main subpopulation of lamina propria T lymphocytes (T-LPL) showed a T-helper phenotype and a smaller subpopulation a suppressor/cytotoxic phenotype. Practically all T-LPL expressed the pan T marker W3/13, and half of these T cells were MRC OX-19+. The results proved to be in agreement with the data obtained after enzymic or mechanical isolation procedures and indicate that proportional contamination with LPL is not likely to occur with these methods.
对大鼠肠黏膜中的淋巴细胞亚群进行原位定量，并与其他作者使用酶法或机械法（Selby等人，1984年；Gibson等人，1985年）获得的数据进行比较，以评估这些酶法或机械分离程序后是否存在任何细胞类型的选择性损失或固有层淋巴细胞的污染。上皮内淋巴细胞（IEL）主要具有抑制/细胞毒性表型（MRC OX-8），几乎所有细胞都带有泛T标志物W3/13。约10%的IEL在表型上属于T辅助细胞（W3/25）谱系。MRC OX-19，相当于小鼠Lyt-1抗原的大鼠抗原，存在于约15%的IEL上。固有层T淋巴细胞（T-LPL）的主要亚群表现出T辅助细胞表型，较小的亚群表现出抑制/细胞毒性表型。几乎所有的T-LPL都表达泛T标志物W3/13，其中一半的T细胞为MRC OX-19阳性。结果证明与酶法或机械分离程序后获得的数据一致，表明这些方法不太可能发生LPL的比例性污染。
