Zhang Jiyue, Zhou Chuang, Tan Maoling, Cao Yanan, Ren Yuanhang, Peng Lianxin
Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu 610106, China.
Foods. 2024 Aug 21;13(16):2624. doi: 10.3390/foods13162624.
Plant-derived edible nanovesicles serve as crucial nanocarriers for targeted delivery of bioactive substances, including miRNAs and phytochemicals, to specific tissues. They have emerged as a significant focus in precision nutrient delivery research. In this study, Tartary-buckwheat-derived nanoparticles (TBDNs) were isolated and purified using a combination of differential centrifugation and PEG precipitation. A response surface test was employed to optimize the extraction process of TBDNs in terms of yield, total phenol and flavonoid content, as well as antioxidant activity. The results demonstrated that TBDNs exhibited the highest yield and activity at a 10% concentration of PEG, pH 5, and centrifugation temperature of 4 °C. Under these conditions, the measured yield of TBDNs was 1.7795 g/kg, with a total phenol content of 178.648 mg/100 g, total flavonoid content of 145.421 mg/100 g, and DPPH-radical-scavenging rate reaching 86.37%. Characterization through a transmission electron microscope and nanoparticle-size-tracking analyzer revealed that TBDNs possessed a teato-type vesicle structure with dispersed vesicle clusters present within them. Furthermore, the extracted TBDNs were found to have an average particle size of 182.8 nm with the main peak observed at 162.8 nm when tested for particle size distribution analysis. These findings provide a novel method for extracting TBDNs while laying the groundwork for future investigations into their activities.
植物源可食用纳米囊泡是将生物活性物质（包括微小RNA和植物化学物质）靶向递送至特定组织的关键纳米载体。它们已成为精准营养递送研究的一个重要焦点。在本研究中，采用差速离心和聚乙二醇沉淀相结合的方法分离并纯化了苦荞源纳米颗粒（TBDNs）。采用响应面试验从产量、总酚和黄酮含量以及抗氧化活性方面优化TBDNs的提取工艺。结果表明，在聚乙二醇浓度为10%、pH值为5、离心温度为4℃的条件下，TBDNs的产量和活性最高。在此条件下，测得的TBDNs产量为1.7795 g/kg，总酚含量为178.648 mg/100 g，总黄酮含量为145.421 mg/100 g，DPPH自由基清除率达到86.37%。通过透射电子显微镜和纳米颗粒尺寸追踪分析仪进行表征，结果显示TBDNs具有茶托型囊泡结构，内部存在分散的囊泡簇。此外，在进行粒径分布分析测试时，发现提取的TBDNs平均粒径为182.8 nm，主峰出现在162.8 nm处。这些发现为提取TBDNs提供了一种新方法，同时为未来对其活性的研究奠定了基础。
