Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Province Engineering Technology Research Center of Coarse Cereal Industralization, Chengdu University, Chengdu 610106, People's Republic of China.
State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People's Republic of China.
J Agric Food Chem. 2022 Mar 2;70(8):2616-2629. doi: 10.1021/acs.jafc.1c07658. Epub 2022 Feb 15.
Evidence suggests that plant-derived nanovesicles may play a significant role in human health. Tartary buckwheat has several physiological activities; however, its underlying health-promoting mechanism remains unclear. In this study, first, Tartary buckwheat-derived nanovesicles (TBDNs) were collected, their structures were analyzed, and microRNA sequencing was performed. Next, target prediction and functional verification were conducted. Finally, the effects of TBDNs on gut microbiota and short-chain fatty acid levels were evaluated. The average size of TBDNs was 141.8 nm diameter. Through the sequencing analyses, 129 microRNAs, including 11 novel microRNAs were identified. Target gene prediction showed that some microRNAs could target functional genes in and -related physiological processes. TBDNs significantly promoted the growth of and , enhanced the diversity of fecal microorganisms and increased the short-chain fatty acid levels. These findings provided a new nutritional perspective for Tartary buckwheat and were conducive to promote the development and utilization of Tartary buckwheat.
有证据表明，植物来源的纳米囊泡可能在人类健康中发挥重要作用。苦荞具有多种生理活性，但其潜在的促进健康的机制尚不清楚。在这项研究中，首先收集了苦荞来源的纳米囊泡（TBDN），分析了它们的结构，并进行了 microRNA 测序。然后进行了靶基因预测和功能验证。最后，评估了 TBDN 对肠道微生物群和短链脂肪酸水平的影响。TBDN 的平均直径为 141.8nm。通过测序分析，共鉴定出 129 个 microRNAs，包括 11 个新的 microRNAs。靶基因预测表明，一些 microRNAs 可以靶向 和 -相关生理过程中的功能基因。TBDN 显著促进了 和 的生长，增强了粪便微生物的多样性，并增加了短链脂肪酸的水平。这些发现为苦荞提供了一个新的营养视角，有助于促进苦荞的开发和利用。
