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酶联免疫吸附测定(ELISA)的自动化

Automation of enzyme-linked immunosorbent assay (ELISA).

作者信息

Carlier Y, Bout D, Capron A

出版信息

J Immunol Methods. 1979;31(3-4):237-46. doi: 10.1016/0022-1759(79)90136-4.

Abstract

A prototype of automatized enzyme-linked immunosorbent assay (ELISA) in tubes is described, using a commercially available basic material, easily modified. Nine hundred samples could be completely studied in a day by only one person. The different steps of the automatized ELISA were systematically studied to obtain the best performance. Its application is described in toxoplasmosis serodiagnosis.

摘要

本文描述了一种试管内自动酶联免疫吸附测定(ELISA)的原型,它使用市售的基础材料,易于改装。仅一人一天就能完全检测900个样本。对自动ELISA的不同步骤进行了系统研究以获得最佳性能。其在弓形虫病血清学诊断中的应用也有描述。

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