• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酶联免疫吸附测定（ELISA）及其微适配技术在弓形虫病血清学诊断中的应用。

Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis.

作者信息

Walls K W, Bullock S L, English D K

出版信息

J Clin Microbiol. 1977 Mar;5(3):273-7. doi: 10.1128/jcm.5.3.273-277.1977.

DOI:10.1128/jcm.5.3.273-277.1977
PMID:323276
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274581/
Abstract

The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the microtitration method were used successfully. ELISA results are equivalent to those found in the indirect immunofluorescence test, yet the ELISA procedure is simpler and more rapid to perform.

摘要

酶联免疫吸附测定（ELISA）已被证明是一种用于弓形虫病血清学诊断的灵敏且特异的定量检测方法。利用弓形虫模型，对该检测的几个参数进行了研究。在一个两倍稀释范围内，每日重复性为90%，与其他疾病的血清样本进行检测时特异性为98%。试管法和微量滴定法均成功应用。ELISA结果与间接免疫荧光试验的结果相当，但ELISA操作更简单、更快速。

相似文献

1
Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis.酶联免疫吸附测定（ELISA）及其微适配技术在弓形虫病血清学诊断中的应用。
J Clin Microbiol. 1977 Mar;5(3):273-7. doi: 10.1128/jcm.5.3.273-277.1977.
2
An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis.一种用于检测弓形虫IgM抗体的酶联免疫吸附测定法：用于急性获得性弓形虫病的诊断。
J Infect Dis. 1980 Nov;142(5):757-66. doi: 10.1093/infdis/142.5.757.
3
A standardized enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Toxoplasma gondii.一种用于检测弓形虫抗体的标准化酶联免疫吸附测定（ELISA）。
J Immunoassay. 1981;2(1):45-57. doi: 10.1080/01971528108062991.
4
Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera.用于定量检测人血清中弓形虫抗体的酶联免疫吸附测定法。
J Clin Pathol. 1980 Jul;33(7):635-9. doi: 10.1136/jcp.33.7.635.
5
[Enzyme-linked-immunosorbent assay (ELISA) for demonstrating antibodies against toxoplasmosis].用于检测抗弓形虫抗体的酶联免疫吸附测定（ELISA）
Klin Monbl Augenheilkd. 1983 Apr;182(4):314-7. doi: 10.1055/s-2008-1054774.
6
Enzyme-linked immunosorbent assay for the detection of toxoplasmosis in swine: interpreting assay results and comparing with other serologic tests.用于检测猪弓形虫病的酶联免疫吸附测定：分析测定结果并与其他血清学检测方法进行比较
Am J Vet Res. 1984 Sep;45(9):1719-25.
7
Evaluation of the enzyme-linked immunosorbent assay (ELISA) and other serological tests for the diagnosis of toxoplasmosis.酶联免疫吸附测定（ELISA）及其他血清学检测用于诊断弓形虫病的评估。
Bull World Health Organ. 1980;58(1):99-105.
8
[Comparative study of the indirect immunofluorescence test and ELISA (enzyme-linked immunosorbent assay) for toxoplasmosis in 877 sera].877份血清中弓形虫病间接免疫荧光试验与ELISA（酶联免疫吸附测定）的比较研究
Rev Latinoam Microbiol. 1981 Oct-Dec;23(4):235-8.
9
[Use of the ELISA (enzyme-linked immunosorbent assay) method in detection of anti-toxoplasma IgG and IgM].[酶联免疫吸附测定（ELISA）法在检测抗弓形虫IgG和IgM中的应用]
Boll Ist Sieroter Milan. 1981;60(6):498-507.
10
[The enzyme linked immuno-sorbent assay (ELISA) method applied for human toxoplasmosis diagnosis (author's transl)].[酶联免疫吸附测定（ELISA）法用于人类弓形虫病诊断（作者译）]
Pathol Biol (Paris). 1982 Feb;30(2):97-101.

引用本文的文献

1
Importance of serological cross-reactivity among Toxoplasma gondii, Hammondia spp., Neospora spp., Sarcocystis spp. and Besnoitia besnoiti.弓形虫、哈蒙德虫属、新孢子虫属、肉孢子虫属和贝斯诺虫之间血清学交叉反应的重要性。
Parasitology. 2017 Jun;144(7):851-868. doi: 10.1017/S0031182017000063. Epub 2017 Feb 28.
2
Sequential recognition of antigenic markers of Toxoplasma gondii tachyzoite by pooled sera of mice with experimental toxoplasmosis.用实验性弓形虫病小鼠的混合血清对刚地弓形虫速殖子的抗原标记物进行顺序识别。
Parasitol Res. 2011 Jan;108(1):151-60. doi: 10.1007/s00436-010-2046-0. Epub 2010 Sep 22.
3
Enzyme-linked immunosorbent assay for the serodiagnosis of toxoplasmosis.用于弓形虫病血清学诊断的酶联免疫吸附测定
J Clin Pathol. 1980 Sep;33(9):841-4. doi: 10.1136/jcp.33.9.841.
4
ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests.用于弓形虫抗体检测的酶联免疫吸附测定（ELISA）：与其他血清学诊断试验的比较
J Clin Pathol. 1980 Jul;33(7):640-3. doi: 10.1136/jcp.33.7.640.
5
Evaluation of an enzyme-linked immunosorbent assay specific for human immunoglobulin G as a Screening test for detecting anti-toxoplasma antibodies.评估一种针对人免疫球蛋白G的酶联免疫吸附测定法作为检测抗弓形虫抗体的筛查试验。
J Clin Microbiol. 1982 Aug;16(2):367-72. doi: 10.1128/jcm.16.2.367-372.1982.
6
Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.用梅毒螺旋体抗原评估微酶联免疫吸附测定法。
J Clin Microbiol. 1982 Apr;15(4):630-4. doi: 10.1128/jcm.15.4.630-634.1982.
7
Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections.用于传染病诊断的免疫球蛋白M免疫吸附凝集试验：急性先天性和获得性弓形虫感染的诊断
J Clin Microbiol. 1981 Nov;14(5):486-91. doi: 10.1128/jcm.14.5.486-491.1981.
8
Method for avoiding false-positive results occurring in immunoglobulin M enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies.避免由于类风湿因子和抗核抗体同时存在而在免疫球蛋白M酶联免疫吸附测定中出现假阳性结果的方法。
J Clin Microbiol. 1981 Jul;14(1):73-8. doi: 10.1128/jcm.14.1.73-78.1981.
9
Reverse enzyme immunoassay for detection of specific anti-Toxoplasma immunoglobulin M antibodies.用于检测特异性抗弓形虫免疫球蛋白M抗体的反向酶免疫测定法。
J Clin Microbiol. 1981 May;13(5):859-64. doi: 10.1128/jcm.13.5.859-864.1981.
10
Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera.用于定量检测人血清中弓形虫抗体的酶联免疫吸附测定法。
J Clin Pathol. 1980 Jul;33(7):635-9. doi: 10.1136/jcp.33.7.635.

本文引用的文献

1
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.
2
Immunoassay using hapten--enzyme conjugates.使用半抗原-酶缀合物的免疫测定法。
FEBS Lett. 1972 Jul 15;24(1):77-81. doi: 10.1016/0014-5793(72)80830-5.
3
Determinations of proteins and hormones in serum by an immunoassay using antigen-enzyme conjugates.使用抗原 - 酶偶联物的免疫测定法测定血清中的蛋白质和激素。
Clin Chim Acta. 1972 Aug;40(1):187-92. doi: 10.1016/0009-8981(72)90269-0.
4
Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays.用酶联免疫吸附测定法对猪旋毛虫感染进行血清学诊断。
Bull World Health Organ. 1974;51(1):108-9.
5
Peroxidase-labeled antibody. A new method of conjugation.过氧化物酶标记抗体。一种新的偶联方法。
J Histochem Cytochem. 1974 Dec;22(12):1084-91. doi: 10.1177/22.12.1084.
6
Letter: Application of ELISA--enzyme linked immunosorbent assay--in the sero-diagnosis of amoebiasis.信函：酶联免疫吸附测定法（ELISA）在阿米巴病血清诊断中的应用。
Trans R Soc Trop Med Hyg. 1975;69(4):440. doi: 10.1016/0035-9203(75)90202-3.
7
Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection.免疫荧光和免疫酶方法在旋毛虫感染血清学诊断中的应用。
Ann N Y Acad Sci. 1975 Jun 30;254:296-303. doi: 10.1111/j.1749-6632.1975.tb29179.x.
8
New serological test for malaria antibodies.疟疾抗体新血清学检测方法。
Br Med J. 1975 Mar 22;1(5959):659-61. doi: 10.1136/bmj.1.5959.659.
9
A microplate enzyme-immunoassay for toxoplasma antibody.一种用于检测弓形虫抗体的微孔板酶免疫测定法。
J Clin Pathol. 1976 Feb;29(2):150-3. doi: 10.1136/jcp.29.2.150.
10
Quantitation of Salmonella O-antibodies in human sera by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定法（ELISA）对人血清中的沙门氏菌O抗体进行定量分析。
Int Arch Allergy Appl Immunol. 1975;48(4):485-94. doi: 10.1159/000231336.