酶联免疫吸附测定（ELISA）及其微适配技术在弓形虫病血清学诊断中的应用。
Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis.
作者信息
Walls K W, Bullock S L, English D K
出版信息
J Clin Microbiol. 1977 Mar;5(3):273-7. doi: 10.1128/jcm.5.3.273-277.1977.
Abstract
The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the microtitration method were used successfully. ELISA results are equivalent to those found in the indirect immunofluorescence test, yet the ELISA procedure is simpler and more rapid to perform.
摘要
酶联免疫吸附测定（ELISA）已被证明是一种用于弓形虫病血清学诊断的灵敏且特异的定量检测方法。利用弓形虫模型，对该检测的几个参数进行了研究。在一个两倍稀释范围内，每日重复性为90%，与其他疾病的血清样本进行检测时特异性为98%。试管法和微量滴定法均成功应用。ELISA结果与间接免疫荧光试验的结果相当，但ELISA操作更简单、更快速。
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