Some characteristics of a new multiple-channel photometer for through-the-plate reading of microplates to be used in enzyme-linked immunosorbent assay.
作者信息
Ruitenberg E J, Sekhuis V M, Brosi B J
出版信息
J Clin Microbiol. 1980 Feb;11(2):132-4. doi: 10.1128/jcm.11.2.132-134.1980.
Abstract
The performance of a prototype of a now commercially available multiple-channel photometer for high-speed reading of microplates to be used in the enzyme-linked immunosorbent assay was evaluated. For this purpose the extinction of a stable test liquid dispensed in flat-bottomed polystyrene microplates was measured, varying the test volume and the optical density. From the results obtained for dilutions of a single, biolgoically inactive material we concluded that the photometric inaccuracy is negligible when performing enzyme-linked immunosorbent assay with biologically active samples.
摘要
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