Faculty of Agriculture, Shahrood University of Technology, Shahrood 3619995161, Iran.
USDA Forest Service, Northern Research Station, Hardwood Tree Improvement and Regeneration Center (HTIRC), Department of Forestry and Natural Resources, Purdue University, 715 West State Street, West Lafayette, IN 47906, USA.
Genes (Basel). 2023 Jan 27;14(2):333. doi: 10.3390/genes14020333.
Sulfate transporters (SULTRs) are an essential plant transporter class responsible for the absorption and distribution of sulfur, an essential plant growth element. SULTRs are also involved in processes related to growth and development and in response to environmental stimuli. In the present study, 22 SULTR family members were identified and characterized in the genome of L. ssp. (Desf.) using available bioinformatics tools. The expression levels of candidate genes were investigated under salt treatments of 150 and 250 mM NaCl after several different exposure times. SULTRs showed diversity in terms of physiochemical properties, gene structure, and pocket sites. SULTRs and their orthologues were classified into the known five main plant groups of highly diverse subfamilies. In addition, it was noted that segmental duplication events could lengthen SULTR family members under evolutionary processes. Based on pocket site analysis, the amino acids leucine (L), valine (V), and serine (S) were most often detected in SULTR protein binding sites. Moreover, it was predicted that SULTRs have a high potential to be targeted by phosphorylation modifications. According to promoter site analysis, the plant bioregulators ABA and MeJA were predicted to affect expression patterns. Real-time PCR analysis revealed genes are differentially expressed at 150 mM NaCl but show similar expression in response to 250 mM NaCl. reached a maximum level of expression 72 h after the 250 mM salt treatment. Overall, we conclude that genes are involved in the response to salinity in durum wheat. However, additional studies of functionality are needed to determine their precise function and linked-interaction pathways.
硫酸盐转运蛋白（SULTRs）是一类重要的植物转运蛋白，负责吸收和分配硫，硫是植物生长的必需元素。SULTRs 还参与与生长发育相关的过程，并对环境刺激做出响应。本研究利用已有的生物信息学工具，在 L. ssp. （Desf.）基因组中鉴定并表征了 22 个 SULTR 家族成员。在不同暴露时间后，用 150 和 250 mM NaCl 进行盐处理，研究了候选基因的表达水平。SULTRs 在理化性质、基因结构和口袋位点方面表现出多样性。SULTRs 及其同源物被分为已知的五个主要植物组，具有高度多样化的亚家族。此外，还注意到在进化过程中，片段重复事件可能会使 SULTR 家族成员变长。根据口袋位点分析，苏氨酸（L）、缬氨酸（V）和丝氨酸（S）这三种氨基酸最常被检测到 SULTR 蛋白结合位点。此外，预测 SULTRs 很有可能成为磷酸化修饰的靶标。根据启动子位点分析，预测植物生物调节剂 ABA 和 MeJA 可能会影响 表达模式。实时 PCR 分析显示，在 150 mM NaCl 下， 基因的表达水平不同，但对 250 mM NaCl 的响应相似。在 250 mM 盐处理后 72 小时， 基因达到最大表达水平。总的来说，我们得出结论， 基因参与了硬粒小麦对盐度的响应。然而，需要进一步的功能研究来确定它们的确切功能和相关的相互作用途径。
