Department of Oral and Maxillofacial Surgery, Dental and Life Science Institute, Dental School, Pusan National University, Busan, South Korea.
Department of Research and Development, FEAGLE Corporations, 70‑6, Jeungsan‑ro, Mulgeum‑eup, Yangsan‑si, Gyeongsangnam‑do 50614, South Korea.
Int J Med Sci. 2022 Sep 25;19(12):1732-1742. doi: 10.7150/ijms.77484. eCollection 2022.
This experimental research aimed to determine whether No-ozone Cold Plasma (NCP) has regenerative effect on crushed injured sensory nerves in a rat model (Wistar A) and to evaluate whether NCP can be used as an alternative treatment method for sensory nerve injury in the oral-maxillofacial region. A total of 10 Wistar A rats were used for this experiment. They were divided into three groups according to whether the mental nerve of the left mandible was injured and NCP was applied or not: group 1 (n=3) (non-mental nerve damage, non-MD) - the left mental nerve was exposed and non-damaged; group 2 (n=3) (mental nerve damage, MD) - the left mental nerve was exposed and damaged, NCP was not applied; and group 3 (n=4) (mental nerve damage and NCP, MD-NCP) - the left mental nerve was exposed and damaged, NCP was applied with regular intervals (three times a week). For the behavior analysis, von Frey test was used. Furthermore, the nerve tissues were examined with hematoxylin and eosin (H&E) staining, and the extent of neurorecovery was evaluated with the immunofluorescence staining of certain markers. The behavioral analysis showed that the function recovery sensory nerve was faster in group 3 (MD-NCP). In the histomorphologic and immunofluorescence analyses, the expression of the factors involved in neurorecovery was much higher in group 3 than in group 2 (MD). The expeditious recovery of sensory nerve function as well as the higher expression of the factors indicating nerve function recovery in the NCP-treated group suggest that NCP has a positive effect on regeneration after sensory nerve crushing injury. Therefore, in the case of sensory impairment of the oral-maxillofacial region, no-ozone cold plasma can be applied for therapeutic effect.
本实验研究旨在确定无臭氧冷等离子体（NCP）是否对大鼠模型中粉碎性损伤感觉神经具有再生作用，并评估 NCP 是否可用作口腔颌面感觉神经损伤的替代治疗方法。 该实验共使用 10 只 Wistar A 大鼠。根据左侧下颌骨颏神经是否损伤以及是否应用 NCP，将它们分为三组：组 1（n=3）（非颏神经损伤，非-MD）-暴露但不损伤左侧颏神经；组 2（n=3）（颏神经损伤，MD）-暴露并损伤左侧颏神经，未应用 NCP；组 3（n=4）（颏神经损伤和 NCP，MD-NCP）-暴露并损伤左侧颏神经，有规律地（每周三次）应用 NCP。 对于行为分析，使用 von Frey 测试。此外，通过苏木精和伊红（H&E）染色检查神经组织，并通过特定标志物的免疫荧光染色评估神经恢复程度。行为分析显示，组 3（MD-NCP）的感觉神经功能恢复更快。在组织形态学和免疫荧光分析中，组 3（MD-NCP）中与神经恢复相关的因子表达明显高于组 2（MD）。 NCP 治疗组感觉神经功能恢复迅速，提示 NCP 对粉碎性损伤后神经再生具有积极作用。因此，在口腔颌面感觉功能障碍的情况下，可应用无臭氧冷等离子体进行治疗。
