Islam Sufia, Nasrin Nishat, Tithi Nigar Sultana, Khatun Farjana, Asaduzzaman Muhammad, Topa Anika Fatema, Kabir Md Farhad, Haque Fahim Kabir Monjurul, Jubair Mohammad, Rahman Mustafizur, Lehmann Christian
Department of Pharmacy, East West University, Dhaka 1212, Bangladesh.
Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka 1000, Bangladesh.
Life (Basel). 2024 Aug 2;14(8):971. doi: 10.3390/life14080971.
Children carrying in their nasopharynx are at a higher risk of contracting systemic infection. Due to lack of sufficient information regarding such carriage, this study was conducted to explore the prevalence, antibiotic susceptibility, and genomic profiles of isolated from nasopharyngeal samples of 163 randomly selected asymptomatic Bangladeshi children aged from 5-<15 years. Antibiotic susceptibility pattern and genomic analysis of the samples were conducted using standard microbiological methods and genomic tools. The carriage was confirmed in 44 (27%) children who were mostly well nourished without respiratory symptoms in the last 3 months. Higher carriage was observed among the younger age group (5-<10 years) who completed vaccines for pneumonia ( = 0.002) and influenza ( = 0.004). Among the isolates, 84.1% were multidrug-resistant and 47.5% (n = 40) were methicillin-resistant (MRSA). All the isolates (100%) were resistant to cefixime with higher resistance to ampicillin (95.5%) and penicillin (90.9%). Among the three investigated isolates, two were ST80 (ID-1 and ID-52) and one was a novel strain (ID-19) with the presence of -S, , , , , , , , and 38 genes. The current research demonstrates a high incidence of multidrug-resistant and reports the first instance of ST80 in asymptomatic children in Bangladesh.
携带于鼻咽部的儿童发生全身感染的风险更高。由于缺乏关于这种携带情况的足够信息，本研究旨在探讨从163名年龄在5至<15岁之间随机选取的无症状孟加拉国儿童的鼻咽样本中分离出的[具体细菌名称未给出]的流行率、抗生素敏感性和基因组特征。使用标准微生物学方法和基因组工具对样本进行抗生素敏感性模式和基因组分析。在44名（27%）儿童中证实存在携带情况，这些儿童大多营养良好，在过去3个月内无呼吸道症状。在完成肺炎疫苗（P = 0.002）和流感疫苗（P = 0.004）接种的较年轻年龄组（5至<10岁）中观察到更高的携带率。在分离株中，84.1%对多种药物耐药，47.5%（n = 40）对甲氧西林耐药[具体细菌名称未给出]（MRSA）。所有分离株（100%）对头孢克肟耐药，对氨苄西林（95.5%）和青霉素（90.9%）的耐药性更高。在三个研究的分离株中，两个是ST80（ID - 1和ID - 52），一个是新菌株（ID - 19），存在[具体基因名称未给出]-S、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和38个基因。当前研究表明多重耐药[具体细菌名称未给出]的发生率很高，并报告了孟加拉国无症状儿童中首次出现ST80的情况。
