Research Centre for Vegetables and Ornamental Crops, Council for Agricultural Research and Economics (CREA), Via dei Fiori 8, 51017 Pescia, Italy.
School of Natural Sciences, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON P3E 2C6, Canada.
Molecules. 2024 Aug 10;29(16):3803. doi: 10.3390/molecules29163803.
L. (Brassicaceae) is an ornamental plant newly identified in Europe as a promising industrial oilseed crop for its valuable very-long-chain monounsaturated fatty acids (MUFAs), especially erucic acid (EA) and nervonic acid (NA). seeds were obtained from annual winter-type plants selected and cultivated in Northern France. Using a systematic multiple-method approach, we set out to determine the profile and content of glucosinolates (GSLs), which are the relevant chemical tag of Brassicaceae. Intact GSLs were analyzed through a well-established LC-MS method. Identification and quantification were performed by HPLC-PDA of desulfo-GSLs (dGLs) according to the official EU ISO method. Moreover, GSL structures were confirmed by GC-MS analysis of the related isothiocyanates (ITCs). Seven GSLs were identified, directly or indirectly, as follows: 1-methylethyl GSL, (1)-1-methylpropyl GSL, ()-5-(methylsulfinyl)pentyl GSL, ()-6-(methylsulfinyl)hexyl GSL, (2)-2-hydroxy-4-pentenyl GSL, 2-phenylethyl GSL, and 1-methoxyindol-3-ylmethyl GSL. In other respects, the FA composition of the seed oil was determined. Results revealed cultivated seed to be a source of NA-rich oil, and presscake as a valuable coproduct. This presscake is indeed rich in GSLs (4.3% /), precursors of promising bioactive molecules for agricultural and nutraceutical applications.
（芸薹属）是一种在欧洲新发现的观赏性植物，作为一种有前途的工业油籽作物，因其具有宝贵的超长链单不饱和脂肪酸（MUFA），特别是芥酸（EA）和神经酸（NA）而备受关注。种子来自于在法国北部选择和种植的一年生冬季型植物。我们采用系统的多方法方法，旨在确定作为芸薹属相关化学标记的硫代葡萄糖苷（GSLs）的特征和含量。通过建立良好的 LC-MS 方法分析完整的 GSL。根据欧盟 ISO 官方方法，通过 HPLC-PDA 对脱硫硫代葡萄糖苷（dGLs）进行鉴定和定量。此外，通过相关异硫氰酸酯（ITC）的 GC-MS 分析确认 GSL 结构。以直接或间接的方式鉴定出以下七种 GSL：1-乙基 GSL、(1)-1-丙基 GSL、()-5-（甲基亚磺酰基）戊基 GSL、()-6-（甲基亚磺酰基）己基 GSL、(2)-2-羟基-4-戊烯基 GSL、2-苯乙基 GSL 和 1-甲氧基吲哚-3-基甲基 GSL。在其他方面，种子油的脂肪酸组成也被确定。结果表明，栽培的种子是富含 NA 的油的来源，而饼粕则是一种有价值的副产物。这种饼粕确实富含 GSL（4.3%/），是农业和营养保健品应用中具有前景的生物活性分子的前体。
