Drug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Pharmaceutical Botany Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via Irnerio 42, 40127 Bologna, Italy.
Molecules. 2024 Aug 11;29(16):3811. doi: 10.3390/molecules29163811.
The development of innovative products for restoring skin integrity and promoting wound healing is still a challenge. The aim of this work was to evaluate an innovative Spanish broom wound dressing impregnated with extract-loaded ufasomes to improve wound healing. Ufasomes were characterized in terms of size, polydispersity index, entrapment efficiency, zeta potential, and stability. In addition, in vitro release studies and biocompatibility, biosafety, and scratch tests on WS1 fibroblasts were performed. The loaded ufasomes showed a nanometric size (<250 nm), good size distribution (lower than 0.3), and appropriate encapsulation efficiency (~67%). Moreover, the lipid vesicles showed good stability during the storage period and allowed for a slow release of glycyrrhizin, the main bioactive compound of the extract. Biological studies revealed that loaded vesicles are not cytotoxic, are hemocompatible, and lead to the complete closure of the scratch after about 33 h. To conclude, the results suggest that the developed dressings can be efficiently used to promote the healing process.
创新产品的开发对于恢复皮肤完整性和促进伤口愈合仍然是一个挑战。本工作的目的是评估一种新型西班牙金雀花伤口敷料，其浸渍有负载提取物的 Ufasomes 以改善伤口愈合。Ufasomes 的特性包括粒径、多分散指数、包封效率、Zeta 电位和稳定性。此外，还进行了体外释放研究以及 WS1 成纤维细胞的生物相容性、生物安全性和划痕试验。负载的 Ufasomes 具有纳米级尺寸（<250nm）、良好的粒径分布（低于 0.3）和适当的包封效率（~67%）。此外，脂质体在储存期间表现出良好的稳定性，并允许甘草酸的缓慢释放，甘草酸是提取物的主要生物活性化合物。生物学研究表明，负载的囊泡无细胞毒性、具有血液相容性，并在大约 33 小时后导致划痕完全闭合。总之，结果表明，所开发的敷料可有效地用于促进愈合过程。
