Drug Delivery Research Lab., Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Pharmaceutical Botany Lab., Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via Irnerio 42, 40127 Bologna, Italy.
Molecules. 2022 May 8;27(9):3025. doi: 10.3390/molecules27093025.
Valorization of wild plants to obtain botanical ingredients could be a strategy for sustainable production of cosmetics. This study aimed to select the rosehip extract containing the greatest amounts of bioactive compounds and to encapsulate it in vesicular systems capable of protecting their own antioxidant activity. Chemical analysis of L. extracts was performed by LC-DAD-MS/MS and H-NMR and vitamins, phenolic compounds, sugars, and organic acids were detected as the main compounds of the extracts. Liposomes, prepared by the film hydration method, together with hyalurosomes and ethosomes, obtained by the ethanol injection method, were characterized in terms of vesicle size, polydispersity index, entrapment efficiency, zeta potential, in vitro release and biocompatibility on WS1 fibroblasts. Among all types of vesicular systems, ethosomes proved to be the most promising nanocarriers showing nanometric size (196 ± 1 nm), narrow polydispersity (0.20 ± 0.02), good entrapment efficiency (92.30 ± 0.02%), and negative zeta potential (-37.36 ± 0.55 mV). Moreover, ethosomes showed good stability over time, a slow release of polyphenols compared with free extract, and they were not cytotoxic. In conclusion, ethosomes could be innovative carriers for the encapsulation of rosehip extract.
野生植物的开发利用可以获得植物成分，这可能是化妆品可持续生产的一种策略。本研究旨在选择含有大量生物活性化合物的玫瑰果提取物，并将其封装在能够保护自身抗氧化活性的囊泡系统中。采用 LC-DAD-MS/MS 和 H-NMR 对 L.提取物进行化学分析，检测到维生素、酚类化合物、糖和有机酸是提取物的主要成分。通过薄膜水化法制备脂质体，通过乙醇注入法制备透明质酸囊泡和醇质体，从囊泡大小、多分散指数、包封效率、Zeta 电位、体外释放和 WS1 成纤维细胞的生物相容性等方面对其进行了表征。在所有类型的囊泡系统中，醇质体被证明是最有前途的纳米载体，具有纳米级尺寸（196 ± 1nm）、窄多分散性（0.20 ± 0.02）、良好的包封效率（92.30 ± 0.02%）和负 Zeta 电位（-37.36 ± 0.55 mV）。此外，醇质体具有良好的时间稳定性，与游离提取物相比，多酚释放缓慢，且无细胞毒性。总之，醇质体可以作为玫瑰果提取物的新型封装载体。
