Laboratory of Metabolomics and Isotope Analyses, Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, 603 00 Brno, Czech Republic.
Department of Environmental Engineering, Institute of Chemical Process Fundamentals, Czech Academy of Sciences, Rozvojová 2/135, 165 02 Prague, Czech Republic.
Molecules. 2024 Aug 13;29(16):3840. doi: 10.3390/molecules29163840.
Stilbenes in the roots of and were studied. Root samples were extracted with 100% methanol and analyzed by HPLC and LC-MS. In this way, -resveratrol dimers (/ 455 Da [M + H]), trimers (/ 681 Da [M + H]) and tetramers (/ 907 Da [M + H]) were identified in the extracts. Using LC-NMR in stop-flow mode, pallidol and -ε-viniferin as dimers were identified. After the separation of individual peaks and their measurement by H NMR, and -miyabenol A as a tetramer and -miyabenol C as a trimer were identified. In the case of miyabenol A, it is a chromatographically inseparable mixture of and isomers in the ratio of 2:3 according to H NMR measurement. In the case of -miyabenol C, the -miyabenol C configuration was confirmed. The remaining unidentified peak with a practically identical UV-VIS spectrum to that of -miyabenol C is most likely another isomer of miyabenol C.
研究了 和 根中的芪类化合物。用 100%甲醇提取根样品，并通过 HPLC 和 LC-MS 进行分析。通过这种方式，在提取物中鉴定出 -白藜芦醇二聚体（/ 455 Da [M + H]）、三聚体（/ 681 Da [M + H]）和四聚体（/ 907 Da [M + H]）。使用停流模式下的 LC-NMR，鉴定出二聚体为 Pallidol 和 -ε-viniferin。分离各个峰并通过 1 H NMR 进行测量后，鉴定出四聚体为 -miyabenol A 和三聚体为 -miyabenol C。对于 miyabenol A，根据 1 H NMR 测量，它是 2:3 比例的异构体混合物，在色谱上无法分离。对于 -miyabenol C，确认了 -miyabenol C 的构型。其余未鉴定的峰与 -miyabenol C 的 UV-VIS 光谱几乎相同，很可能是 miyabenol C 的另一种异构体。
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