Department of Biology, University of Osijek, Cara Hadrijana 8A, 31000 Osijek, Croatia.
Department of Chemistry, University of Osijek, Cara Hadrijana 8A, 31000 Osijek, Croatia.
Molecules. 2024 Aug 22;29(16):3961. doi: 10.3390/molecules29163961.
Green synthesis employs environmentally friendly, biodegradable substances for the production of nanomaterials. This study aims to develop an innovative method for synthesizing silver nanoparticles (AgNPs) using a methanolic extract of L. Fr. as the reducing agent and to assess the potential antibacterial properties of the resulting nanoparticles. The successful synthesis of AgNPs was confirmed through characterization techniques such as UV-visible (UV-Vis) spectrophotometry, Fourier-transform infrared spectroscopy (FT-IR), and powder X-ray diffraction (PXRD). The UV-Vis analysis revealed an absorption peak at 423 nm, while FT-IR identified key phytochemical compounds involved in the reduction process. PXRD analysis indicated a face-centered cubic (fcc) structure with prominent peaks observed at 2θ = 38°, 44.6°, 64.6°, and 78°, confirming the crystalline nature of the AgNPs, with a crystallite diameter of approximately 24 nm, consistent with TEM analysis. The synthesized AgNPs demonstrated significant antibacterial activity, particularly against , with higher efficacy against gram-positive bacteria.
绿色合成采用环保、可生物降解的物质来生产纳米材料。本研究旨在开发一种使用 L. Fr. 的甲醇提取物作为还原剂来合成银纳米粒子 (AgNPs) 的创新方法，并评估所得纳米粒子的潜在抗菌性能。通过紫外-可见 (UV-Vis) 分光光度法、傅里叶变换红外光谱 (FT-IR) 和粉末 X 射线衍射 (PXRD) 等表征技术证实了 AgNPs 的成功合成。UV-Vis 分析显示在 423nm 处有一个吸收峰，而 FT-IR 则鉴定出参与还原过程的关键植物化学化合物。PXRD 分析表明具有面心立方 (fcc) 结构，在 2θ = 38°、44.6°、64.6°和 78°处观察到明显的峰，证实了 AgNPs 的结晶性质，其晶粒度约为 24nm，与 TEM 分析一致。合成的 AgNPs 表现出显著的抗菌活性，特别是对 ，对革兰氏阳性菌的效果更高。
