Aminmansour Sahar, Cardoso Lais M, Anselmi Caroline, de Carvalho Ana Beatriz Gomes, Rahimnejad Maedeh, Bottino Marco C
Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, 1011 N. University Avenue, Ann Arbor, MI 48109, USA.
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), 1680 Humaitá Street, Araraquara 14801-903, SP, Brazil.
Materials (Basel). 2024 Aug 7;17(16):3904. doi: 10.3390/ma17163904.
This study investigated gelatin methacryloyl (GelMA) and polycaprolactone (PCL) blend scaffolds incorporating cerium oxide (CeO) nanoparticles at concentrations of 0%, 5%, and 10% via electrospinning for periodontal tissue engineering. The impact of photocrosslinking on these scaffolds was evaluated by comparing crosslinked (C) and non-crosslinked (NC) versions. Methods included Fourier transform infrared spectroscopy (FTIR) for chemical analysis, scanning electron microscopy (SEM) for fiber morphology/diameters, and assessments of swelling capacity, degradation profile, and biomechanical properties. Biological evaluations with alveolar bone-derived mesenchymal stem cells (aBMSCs) and human gingival fibroblasts (HGFs) encompassed tests for cell viability, mineralized nodule deposition (MND), and collagen production (CP). Statistical analysis was performed using Kruskal-Wallis or ANOVA/post-hoc tests (α = 5%). Results indicate that C scaffolds had larger fiber diameters (250 nm) compared with NC scaffolds (150 nm). NC scaffolds exhibited higher swelling capacities than C scaffolds, while both types demonstrated significant mass loss (~50%) after 60 days ( < 0.05). C scaffolds containing CeO showed increased Young's modulus and tensile strength than NC scaffolds. Cells cultured on C scaffolds with 10% CeO exhibited significantly higher metabolic activity (>400%, < 0.05) after 7 days among all groups. Furthermore, CeO-containing scaffolds promoted enhanced MND by aBMSCs (>120%, < 0.05) and increased CP in 5% CeO scaffolds for both variants (>180%, < 0.05). These findings underscore the promising biomechanical properties, biodegradability, cytocompatibility, and enhanced tissue regenerative potential of CeO-loaded GelMA/PCL scaffolds for periodontal applications.
本研究通过静电纺丝法制备了含0%、5%和10%氧化铈（CeO）纳米颗粒的明胶甲基丙烯酰基（GelMA）和聚己内酯（PCL）共混支架，用于牙周组织工程。通过比较交联（C）和非交联（NC）版本来评估光交联对这些支架的影响。方法包括傅里叶变换红外光谱（FTIR）进行化学分析、扫描电子显微镜（SEM）观察纤维形态/直径，以及评估溶胀能力、降解曲线和生物力学性能。用牙槽骨来源的间充质干细胞（aBMSCs）和人牙龈成纤维细胞（HGFs）进行的生物学评估包括细胞活力测试、矿化结节沉积（MND）和胶原蛋白生成（CP）。使用Kruskal-Wallis或方差分析/事后检验（α = 5%）进行统计分析。结果表明，与NC支架（约150 nm）相比，C支架的纤维直径更大（约250 nm）。NC支架的溶胀能力高于C支架，而两种类型在60天后均表现出显著的质量损失（约50%）（P<0.05）。含CeO的C支架比NC支架表现出更高的杨氏模量和拉伸强度。在所有组中，在含10% CeO的C支架上培养的细胞在7天后表现出显著更高的代谢活性（>400%，P<0.05）。此外，含CeO的支架促进了aBMSCs增强的MND（>120%，P<0.05），并且两种变体在5% CeO支架中CP均增加（>180%，P<0.05）。这些发现强调了负载CeO的GelMA/PCL支架在牙周应用中具有良好的生物力学性能、生物降解性、细胞相容性和增强的组织再生潜力。
