Whole Genome Sequencing of AMR25, an Effective Antagonist Strain against Plant Pathogens.

The most serious problems for cultivated grapes are pathogenic microorganisms, which reduce the yield and quality of fruit. One of the most widespread disease of grapes is "gray mold", caused by the fungus . Some strains of , such as , , and , are known to be active against major post-harvest plant rots. In this study, we showed that the endophytic bacteria strain AMR25 isolated from the leaves of wild grapes Rupr. exhibited antimicrobial activity against grape pathogens, including . The genome of AMR25 has one circular chromosome with a length of 3,909,646 bp. with 3689 open reading frames. Genomic analysis identified ten gene clusters involved in the nonribosomal synthesis of polyketides (macrolactin, bacillene, and difficidin), lipopeptides (surfactin, fengycin, and bacillizin), and bacteriocins (difficidin). Also, the genome under study contains a number of genes involved in root colonization, biofilm formation, and biosynthesis of phytohormones. Thus, the endophytic bacteria strain AMR25 shows great promise in developing innovative biological products for enhancing plant resistance against various pathogens.