Ananev Alexey A, Ogneva Zlata V, Nityagovsky Nikolay N, Suprun Andrey R, Kiselev Konstantin V, Aleynova Olga A
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia.
Microorganisms. 2024 Jul 26;12(8):1533. doi: 10.3390/microorganisms12081533.
The most serious problems for cultivated grapes are pathogenic microorganisms, which reduce the yield and quality of fruit. One of the most widespread disease of grapes is "gray mold", caused by the fungus . Some strains of , such as , , and , are known to be active against major post-harvest plant rots. In this study, we showed that the endophytic bacteria strain AMR25 isolated from the leaves of wild grapes Rupr. exhibited antimicrobial activity against grape pathogens, including . The genome of AMR25 has one circular chromosome with a length of 3,909,646 bp. with 3689 open reading frames. Genomic analysis identified ten gene clusters involved in the nonribosomal synthesis of polyketides (macrolactin, bacillene, and difficidin), lipopeptides (surfactin, fengycin, and bacillizin), and bacteriocins (difficidin). Also, the genome under study contains a number of genes involved in root colonization, biofilm formation, and biosynthesis of phytohormones. Thus, the endophytic bacteria strain AMR25 shows great promise in developing innovative biological products for enhancing plant resistance against various pathogens.
栽培葡萄面临的最严重问题是致病微生物，它们会降低果实的产量和品质。葡萄最普遍的病害之一是由真菌引起的“灰霉病”。某些 菌株，如 、 和 ，已知对主要的采后植物腐烂病具有活性。在本研究中，我们表明从野生葡萄 鲁普雷希特叶中分离出的内生细菌 菌株AMR25对包括 在内的葡萄病原菌具有抗菌活性。AMR25的基因组有一条长度为3,909,646 bp的环状染色体，有3689个开放阅读框。基因组分析确定了十个参与聚酮化合物（大环内酯、杆菌烯和艰难梭菌素）、脂肽（表面活性素、丰原素和杆菌杀菌素）和细菌素（艰难梭菌素）非核糖体合成的基因簇。此外，所研究的基因组包含许多参与根部定殖、生物膜形成和植物激素生物合成的基因。因此，内生细菌 菌株AMR25在开发增强植物对各种病原体抗性的创新生物产品方面显示出巨大潜力。
