Aleynova Olga A, Nityagovsky Nikolay N, Ananev Alexey A, Suprun Andrey R, Ogneva Zlata V, Dneprovskaya Alina A, Beresh Alina A, Tyunin Alexey P, Dubrovina Alexandra S, Kiselev Konstantin V
Laboratory of Biotechnology, Federal Scientific Center of the East 27Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia.
The School of Natural Sciences, Far Eastern Federal University, 690090 Vladivostok, Russia.
Plants (Basel). 2023 Aug 15;12(16):2952. doi: 10.3390/plants12162952.
Many grape endophytic microorganisms exhibit high potential for suppressing the development of grape diseases and stimulating grapevine growth and fitness, as well as beneficial properties of the crop. The microbiome of wild grapevines is a promising source of biocontrol agents, which can be beneficial for domesticated grapevines. Using next-generation sequencing (NGS) and classical microbiology techniques, we performed an analysis of bacterial and fungal endophytic communities of wild grapevines Rupr. and Pulliat growing in the Russian Far East. According to the NGS analysis, 24 and 18 bacterial taxa from the class level were present in and grapevines, respectively. Gammaproteobacteria (35%) was the predominant class of endophytic bacteria in and Alphaproteobacteria (46%) in Three taxa, namely , and were the most common bacterial genera for and . Metagenomic analysis showed the presence of 23 and 22 fungi and fungus-like taxa of class level in and , respectively. The predominant fungal classes were Dothideomycetes (61-65%) and Tremellomycetes (10-11%), while and were the most common fungal genera in and respectively. A comparative analysis of the endophytic communities of and with the previously reported endophytic communities of revealed that the bacterial biodiversity of and was similar in alpha diversity to 's bacterial biodiversity. The fungal alpha diversity of and was statistically different from that of . The beta diversity analysis of bacterial and fungal endophytes showed that samples of formed separate clusters, while samples formed a separate cluster including samples. The data revealed that the endophytic community of bacteria and fungi from wild was richer than that from grapes and cultivated grapes. Therefore, the data obtained in this work could be of high value in the search for potentially useful microorganisms for viticulture.
许多葡萄内生微生物在抑制葡萄病害发展、促进葡萄生长和健康以及展现作物有益特性方面具有很高潜力。野生葡萄的微生物组是生物防治剂的一个有前景的来源，对栽培葡萄可能有益。利用下一代测序（NGS）和经典微生物学技术，我们对生长在俄罗斯远东地区的野生葡萄Rupr.和Pulliat的细菌和真菌内生群落进行了分析。根据NGS分析，Rupr.和Pulliat葡萄中分别存在24个和18个来自纲水平的细菌分类单元。γ-变形菌纲（35%）是Rupr.葡萄中内生细菌的主要纲，而α-变形菌纲（46%）是Pulliat葡萄中的主要纲。三个分类单元，即、和，是Rupr.和Pulliat葡萄中最常见的细菌属。宏基因组分析表明，Rupr.和Pulliat葡萄中分别存在23个和22个来自纲水平的真菌和类真菌分类单元。主要的真菌纲是座囊菌纲（61 - 65%）和银耳纲（10 - 11%），而和分别是Rupr.和Pulliat葡萄中最常见的真菌属。对Rupr.和Pulliat葡萄的内生群落与先前报道的葡萄内生群落进行比较分析发现，Rupr.和Pulliat葡萄的细菌生物多样性在α多样性方面与葡萄的细菌生物多样性相似。Rupr.和Pulliat葡萄的真菌α多样性与葡萄的在统计学上不同。对细菌和真菌内生菌的β多样性分析表明，Rupr.葡萄的样本形成单独的簇，而Pulliat葡萄的样本形成一个包括葡萄样本的单独簇。数据显示，野生葡萄的细菌和真菌内生群落比葡萄和栽培葡萄的更丰富。因此，这项工作中获得的数据在寻找葡萄栽培中潜在有用微生物方面可能具有很高价值。
