Department of Biomedical and Neuromotor Sciences, Alma Mater Studiorum, University of Bologna, 40126 Bologna, Italy.
Biostructures and Biosystems National Institute (INBB), 00136 Rome, Italy.
Nutrients. 2024 Aug 20;16(16):2770. doi: 10.3390/nu16162770.
Dietary intervention is considered a safe preventive strategy to slow down aging. This study aimed to evaluate the protective effects of a commercially available supplement and six simpler formulations against DNA damage in 3D human keratinocytes. The ingredients used are well known and were combined into various formulations to test their potential anti-aging properties. Firstly, we determined the formulations' safe concentration by evaluating cytotoxicity and cell viability through spectrophotometric assays. We then examined the presence of tumor p53 binding protein 1 and phosphorylated histone H2AX foci, which are markers of genotoxicity. The foci count revealed that a 24-h treatment with the supplement did not induce DNA damage, and significantly reduced DNA damage in cells exposed to neocarzinostatin for 2 h. Three of the simpler formulations showed similar results. Moreover, the antioxidant activity was tested using a recently developed whole cell-based chemiluminescent bioassay; results showed that a 24-h treatment with the supplement and three simpler formulations significantly reduced intracellular HO after pro-oxidant injury, thus suggesting their possible antiaging effect. This study's originality lies in the use of a 3D human keratinocyte cell model and a combination of natural ingredients targeting DNA damage and oxidative stress, providing a robust evaluation of their anti-aging potential.
饮食干预被认为是一种安全的预防策略，可以减缓衰老。本研究旨在评估一种市售补充剂和六种更简单配方对 3D 人角质形成细胞 DNA 损伤的保护作用。所用的成分是众所周知的，并将其组合成各种配方以测试其潜在的抗衰老特性。首先，我们通过分光光度法评估细胞毒性和细胞活力来确定配方的安全浓度。然后，我们检查了肿瘤 p53 结合蛋白 1 和磷酸化组蛋白 H2AX 焦点的存在情况，这些都是遗传毒性的标志物。焦点计数显示，补充剂 24 小时处理不会诱导 DNA 损伤，并且显著降低了 2 小时内暴露于新制癌菌素的细胞中的 DNA 损伤。三种更简单的配方也显示出相似的结果。此外，使用最近开发的全细胞化学发光生物测定法测试了抗氧化活性；结果表明，补充剂和三种更简单的配方 24 小时处理可显著减少活性氧损伤后的细胞内 HO，从而提示其可能具有抗衰老作用。本研究的创新性在于使用了 3D 人角质形成细胞模型和针对 DNA 损伤和氧化应激的天然成分组合，对其抗衰老潜力进行了强有力的评估。
