Department of Integrated Biosicences, College of Biomedical and Health Science, Konkuk University, Chungju-si 27478, Chungcheongbuk-do, Korea.
Department of Biomedical Chemistry, College of Biomedical and Health Science, Konkuk University, Chungju-si 27478, Korea.
Molecules. 2021 Sep 13;26(18):5564. doi: 10.3390/molecules26185564.
Oat () is well known for its various health benefits. The protective effect of oat extract against oxidative stress-induced apoptosis in human keratinocytes HaCaT was determined. First, extracts of two varieties of oat, Daeyang and Choyang, were analyzed for fat-soluble antioxidants such as α-tocotrienol, γ-oryzanols, lutein and zeaxanthin using an UPLC system and for antioxidant activity using a DPPH assay. Specifically, an 80% ethanol extract of Daeyang oat ( cv. Daeyang), which had high amounts of antioxidants and potent radical scavenging activity, was further evaluated for protective effect against oxidative stress-induced cell death, intracellular reactive oxygen species levels, the phosphorylation of DNA damage mediating genes such as H2AX, checkpoint kinase 1 and 2, and p53 and the activation of apoptotic genes such as cleaved caspase-3 and 7 and poly (ADP-ribose) polymerase in HaCaT cells. The Daeyang and Choyang oat 80% ethanol extracts had 26.9 and 24.1 mg/100 g γ-oryzanols, 7.69 and 8.38 mg/100 g α-tocotrienol, 1.25 and 0.34 mg/100 g of lutein and 1.20 and 0.17 mg/100 g of zeaxanthin, respectively. The oat 80% ethanol extract treatment ( cv. Daeyang) had a protective effect on oxidative stress-induced cell death in HaCaT cells. In addition, the oat 80% ethanol extracts led to a significant decrease in the intracellular ROS level at a concentration of 50-200 μg/mL, the attenuation of DNA damage mediating genes and the inhibition of apoptotic caspase activities in a dose dependent manner (50-200 μg/mL). Thus, the current study indicates that an oat ( cv. Daeyang) extract rich in antioxidants, such as polyphenols, avenanthramides, γ-oryzanols, tocotrienols and carotenoids, has a protective role against oxidative stress-induced keratinocyte injuries and that oat may a useful source for oxidative stress-associated skin damage.
燕麦（）以其多种健康益处而闻名。研究了燕麦提取物对人角质形成细胞 HaCaT 氧化应激诱导细胞凋亡的保护作用。首先，使用 UPLC 系统分析了大阳和朝阳两种燕麦品种的脂溶性抗氧化剂，如α-生育三烯酚、γ-谷维素、叶黄素和玉米黄质，并使用 DPPH 测定法分析了抗氧化活性。具体而言，对具有高抗氧化剂含量和强大自由基清除活性的大阳燕麦（ cv. 大阳） 80%乙醇提取物进行了进一步评估，以研究其对氧化应激诱导的细胞死亡、细胞内活性氧水平、DNA 损伤介导基因（如 H2AX、检查点激酶 1 和 2 以及 p53）磷酸化以及 HaCaT 细胞中凋亡基因（如 cleaved caspase-3 和 7 和多聚（ADP-核糖）聚合酶）的保护作用。大阳和朝阳燕麦 80%乙醇提取物分别含有 26.9 和 24.1mg/100gγ-谷维素、7.69 和 8.38mg/100gα-生育三烯酚、1.25 和 0.34mg/100g叶黄素和 1.20 和 0.17mg/100g玉米黄质。燕麦 80%乙醇提取物（ cv. 大阳）对 HaCaT 细胞氧化应激诱导的细胞死亡具有保护作用。此外，燕麦 80%乙醇提取物在 50-200μg/ml 浓度下显著降低细胞内 ROS 水平，在 50-200μg/ml 浓度下剂量依赖性减弱 DNA 损伤介导基因，并抑制凋亡 caspase 活性。因此，本研究表明，富含抗氧化剂的燕麦（ cv. 大阳）提取物，如多酚、燕麦酰胺、γ-谷维素、生育三烯酚和类胡萝卜素，对氧化应激诱导的角质形成细胞损伤具有保护作用，燕麦可能是一种与氧化应激相关的皮肤损伤的有用来源。
