Zeng Hongyun, Huang Bingzhi, Xu Linbing, Wu Yuanli
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Tree, Guangzhou 510640, China.
Plants (Basel). 2024 Aug 6;13(16):2173. doi: 10.3390/plants13162173.
Banana ( spp.) is one of the most economically important horticultural crops. There are many types of banana, with differing ploidy (usually diploid, triploid, or tetraploid) and genome types (most containing the A or/and B genome). Currently, observation and genome type detection are commonly used to identify banana germplasm resources. However, observation is tedious, while genome type detection cannot distinguish categories below genome types. It is, therefore, urgent to establish a simple and effective method for identifying banana germplasm resources. This study sequenced and analyzed the ribosomal DNA internal transcribed spacer (ITS) sequences of 62 banana germplasm resources and found that the sequencing peaks, especially the 20 bp region near the 420-bp position (referred to as the 420-bp region), exhibited relatively recognizable and repeatable polymorphism characteristics. Using the 420-bp region as a marker, we were able to quickly distinguish bananas belonging to different genome type groups or different subgroups in the same genome type group. Moreover, it appeared that Sanger sequencing of ITS could be used to identify hybrid banana offspring. In general, ITS sequencing simplifies the classification of banana germplasm resources and has potential application in several areas of improvement.
香蕉（芭蕉属）是最重要的经济园艺作物之一。香蕉有多种类型，其倍性不同（通常为二倍体、三倍体或四倍体），基因组类型也不同（大多数含有A或/和B基因组）。目前，观察和基因组类型检测是鉴定香蕉种质资源常用的方法。然而，观察过程繁琐，而基因组类型检测无法区分基因组类型以下的类别。因此，迫切需要建立一种简单有效的香蕉种质资源鉴定方法。本研究对62份香蕉种质资源的核糖体DNA内转录间隔区（ITS）序列进行了测序和分析，发现测序峰，尤其是420 bp位置附近的20 bp区域（称为420 bp区域），呈现出相对可识别和可重复的多态性特征。以420 bp区域为标记，我们能够快速区分属于不同基因组类型组或同一基因组类型组中不同亚组的香蕉。此外，ITS的桑格测序似乎可用于鉴定杂交香蕉后代。总体而言，ITS测序简化了香蕉种质资源的分类，在多个改良领域具有潜在应用价值。
