Ma Huili, Zhang Chenjie, Niu Tongfei, Chen Meida, Guo Lili, Hou Xiaogai
College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China.
College of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471023, China.
Plants (Basel). 2023 Jun 26;12(13):2453. doi: 10.3390/plants12132453.
In order to explore the release rule of floral volatile substances and the diurnal variation of different flower development stages of 'Fengdan' in potted and ground-planted conditions, dynamic headspace adsorption combined with gas chromatography-mass spectrometry(GC-MS) was used to analyze the dynamic changes in floral volatile components and contents. Quantitative real-time PCR (qRT-PCR) was used to analyze changes in flower fragrance-regulating genes , , and at different flower development stages and a daily change process at the full-blooming stage. The results show that there were differences in aroma components and contents of 'Fengdan' at different flower development stages and different time quantum of every day. There were 25 and 28 aroma components identified in 7 flower development stages of tree peonies planted in pots and in the field, respectively, and 23 and 22 aroma components identified at different time quantum of the day, of which the largest and highest content was alkanes. The main characteristic aroma substances were (E)-β-ocimene, 1,3,5-trimethoxybenzene, 2,4-di-tert-butylphenol, methyl jasmonate, nerol, and cinnamyl alcohol; released amounts of the abovementioned substances varied depending on the development stage and the time of the day. The expression of flower fragrance-controlling genes (, , and ) in tree peonies varied greatly in different conditions. The results of this study provide a valuable resource to investigate floral fragrance formation in tree peonies.
为了探究盆栽和地栽条件下‘凤丹’不同花朵发育阶段的花香挥发物释放规律及日变化，采用动态顶空吸附结合气相色谱 - 质谱联用（GC - MS）技术分析花香挥发成分及含量的动态变化。运用实时荧光定量PCR（qRT - PCR）分析不同花朵发育阶段以及盛花期日变化过程中花香调控基因、和的变化。结果表明，‘凤丹’在不同花朵发育阶段及每天不同时间段的香气成分和含量存在差异。盆栽和地栽牡丹的7个花朵发育阶段分别鉴定出25种和28种香气成分，一天中不同时间段分别鉴定出23种和22种香气成分，其中含量最多、占比最高的是烷烃类。主要特征香气物质为（E）-β-罗勒烯、1,3,5 - 三甲氧基苯、2,4 - 二叔丁基苯酚、茉莉酸甲酯、橙花醇和肉桂醇；上述物质的释放量随发育阶段和一天中的时间而变化。牡丹花香调控基因（、和）在不同条件下的表达差异很大。本研究结果为探究牡丹花香形成提供了有价值的资源。
