Horticulture College, Shenyang Agricultural University, Shenyang, Liaoning, China.
Forestry College, Shenyang Agricultural University, Shenyang, Liaoning, China.
PLoS One. 2020 Apr 2;15(4):e0231117. doi: 10.1371/journal.pone.0231117. eCollection 2020.
Herbaceous peony (Paeonia lactiflora Pall.) is a popular ornamental and medicinal plant. Taking approximately six to seven months, the seeds germination under natural conditions experiences dual dormancies, which seriously affects horticultural cultivation. Few studies have been conducted on exploring both biological and molecular mechanism that regulates dormancy removal process in hypocotyls double dormant plants. Here, we first measured ABA and GA3 content changes at four key dormancy break stages, and then performed transcriptomic analyses to identify the differentially expressed genes (DEGs) using RNA-seq. We subsequently carried out Quantitative real-time PCR (qRT-PCR) to validate RNA-seq data. ABA content decreased during the whole dormancy removal process and GA3 content exhibited decreasing slightly and then increasing trend. RNA sequencing de novo assembly generated a total of 99,577 unigenes. 20,344 unigenes were differentially expressed in the whole dormancy release process. The qPCR results of 54 selected unigenes were consistent with the FPKM values obtained from RNA-seq. Our results summarize a valuable collection of gene expression profiles characterizing the dormancy release process. The DEGs are candidates for functional analyses of genes affecting the dormancy release, which is a precious resource for the on-going physiological and molecular investigation of seeds dormancy removal in other perennial plants.
草本牡丹（Paeonia lactiflora Pall.）是一种受欢迎的观赏和药用植物。在自然条件下，大约需要六到七个月的时间，种子才能经历双重休眠而发芽，这严重影响了园艺栽培。目前很少有研究探索调控下胚轴休眠解除过程的生物学和分子机制。在这里，我们首先在四个关键休眠解除阶段测量 ABA 和 GA3 的含量变化，然后使用 RNA-seq 进行转录组分析以鉴定差异表达基因（DEGs）。随后，我们进行了定量实时 PCR（qRT-PCR）以验证 RNA-seq 数据。ABA 含量在整个休眠解除过程中降低，GA3 含量表现出先略降后升高的趋势。RNA 测序从头组装共产生了 99577 个 unigenes。在整个休眠释放过程中有 20344 个 unigenes差异表达。54 个选定 unigenes 的 qPCR 结果与 RNA-seq 获得的 FPKM 值一致。我们的结果总结了描述休眠解除过程的有价值的基因表达谱集合。DEGs 是影响休眠解除的基因功能分析的候选基因，这是正在进行的其他多年生植物种子休眠解除的生理和分子研究的宝贵资源。
