Ruiz-García Ana Belén, Candresse Thierry, Malagón José, Ruiz-Torres Manuel, Paz Sergio, Pérez-Sierra Ana, Olmos Antonio
Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra CV-315, km 10.7, 46113 Moncada, Valencia, Spain.
UMR BFP, INRAE, University of Bordeaux, CS 20032, 33882 Villenave d'Ornon CEDEX, France.
Plants (Basel). 2024 Aug 17;13(16):2290. doi: 10.3390/plants13162290.
Studies of the virome of olive trees with symptoms of leaf mottling by high-throughput sequencing (HTS) revealed the presence of a new virus. Full coding genome sequences of two isolates were determined and consisted of a single RNA segment of 16,516 nt and 16,489, respectively. The genomic organization contained 10 open reading frames (ORFs) from 5' to 3': ORF1a, ORF1b (RdRp), ORF2 (p22), ORF3 (p7), ORF4 (HSP70h), ORF5 (HSP90h), ORF6 (CP), ORF7 (p19), ORF8 (p12), ORF9 (p23) and ORF10 (p9). Phylogenetic analyses clustered this virus in the genus , family with the closest species being , commonly named olive leaf yellowing-associated virus (OLYaV). However, amino acid sequences of all taxonomically relevant proteins showed, in all cases, a divergence higher than 25% between OLYaV and the new virus, indicating that it represents a new species in the genus for which the common name of olive leaf mottling virus (OLMV) is proposed. This study represents an advance in the genus and provides new insights into the olive virome.
通过高通量测序(HTS)对出现叶片斑驳症状的橄榄树病毒组进行的研究揭示了一种新病毒的存在。测定了两个分离株的完整编码基因组序列,分别由一个16,516 nt和16,489 nt的单一RNA片段组成。基因组结构包含从5'到3'的10个开放阅读框(ORF):ORF1a、ORF1b(RdRp)、ORF2(p22)、ORF3(p7)、ORF4(HSP70h)、ORF5(HSP90h)、ORF6(CP)、ORF7(p19)、ORF8(p12)、ORF9(p23)和ORF10(p9)。系统发育分析将这种病毒归类于 属、 科,与之亲缘关系最近的物种是 ,通常称为橄榄叶黄化相关病毒(OLYaV)。然而,所有分类学相关蛋白的氨基酸序列在所有情况下均显示,OLYaV与新病毒之间的差异高于25%,这表明它代表了 属中的一个新物种,为此提出了橄榄叶斑驳病毒(OLMV)的通用名称。这项研究代表了 属的一项进展,并为橄榄树病毒组提供了新的见解。