Ruiz-García Ana Belén, Candresse Thierry, Canales Celia, Morán Félix, Machado de Oliveira Carlos, Bertolini Edson, Olmos Antonio
Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Spain.
University Bordeaux, INRAE, UMR 1332 Biologie du Fruit et Pathologie, Equipe de Virologie, 33882 Villenave d'Ornon, France.
Plants (Basel). 2020 Sep 27;9(10):1272. doi: 10.3390/plants9101272.
Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP70h gene. Interestingly, a thaumatin-like protein (ORF2), previously reported in other unassigned viruses in the family, persimmon virus B and actidinia virus 1, was identified in the OLYaV genome. Phylogenetic analysis of shared proteins (ORF1a, ORF1b, HSP70h, HSP90h and CP) with all members of the family provides new insight into the taxonomic position of these three closteroviruses and suggests they could represent a new genus in the family.
确定了与叶黄化相关的油橄榄病毒（OLYaV）的基因组结构及其与该科其他成员的系统发育关系。通过对来自巴西一棵35年树龄（品种为Zarzaleña）、表现出叶黄化病和木材变形的油橄榄树的总RNA进行高通量测序，获得了OLYaV的完整编码序列。这是该国关于OLYaV的首次报道。获得了一个16700 nt的基因组序列，包含11个开放阅读框（ORF），代表了完整的病毒编码能力。了解包括编码外壳蛋白基因在内的基因组核苷酸序列，将有助于开发诊断检测方法，目前诊断检测方法仅限于针对HSP70h基因的基于PCR的方法。有趣的是，在OLYaV基因组中鉴定出一种类thaumatin蛋白（ORF2），该蛋白先前在该科其他未分类病毒、柿树病毒B和猕猴桃病毒1中报道过。对与该科所有成员共享的蛋白（ORF1a、ORF1b、HSP70h、HSP90h和CP）进行系统发育分析，为这三种长线形病毒的分类地位提供了新的见解，并表明它们可能代表该科的一个新属。
