Livadariu Oana, Constantin Carmen Gabriela, Boiu-Sicuia Oana-Alina, Dobrin Aurora, Ion Violeta Alexandra
Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Bd. Marasti, 011464 Bucharest, Romania.
Research Center for Studies of Food Quality and Agricultural Products, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Bd. Marasti, 011464 Bucharest, Romania.
Plants (Basel). 2024 Aug 20;13(16):2313. doi: 10.3390/plants13162313.
Considering the current global climate and demographic conditions, combined with the growing demand for food diversification, the need for innovative functional foods that adhere to the principles of the circular economy is becoming clear. Therefore, this research aims to identify an appropriate cultivation system and growth substrate to maintain a high germination rate and produce basil aromatic microplants with superior quality traits that are entirely edible, together with the substrate. Microplants were grown in both aseptic (AS) and non-aseptic (NAS) systems. Both AS and NAS experiments were conducted in vitro using eco-innovative production technology. Moreover, various growth substrates were tested, such as perlite, agar, banana peel, peat, and their combinations. The analyses focused on the germination capacity, morphometric measurements, and biochemical analyses of the microplants. The results showed that the edible agar-based substrate, used in both AS and NAS, increased the germination capacity up to 95.00 ± 0.30%, while peat provided a germination capacity of only 12.07 ± 1.27% under AS conditions and 6.07 ± 0.35% under NAS conditions. Most biochemical analyses indicated that AS conditions are more suitable for basil microplant production, increasing the dry matter content, total phenolic content, total flavonoid content, and total antioxidant capacity compared to NAS conditions. These findings support the adoption of a new eco-innovative technology that provides organic basil microplants, which are fully usable along with the edible agar substrate.
考虑到当前全球气候和人口状况，再加上对食物多样化的需求不断增长，对遵循循环经济原则的创新型功能性食品的需求日益凸显。因此，本研究旨在确定一种合适的栽培系统和生长基质，以保持高发芽率，并生产出具有卓越品质特性且可与基质一起完全食用的罗勒芳香微型植物。微型植物在无菌（AS）和非无菌（NAS）系统中生长。AS和NAS实验均采用生态创新生产技术在体外进行。此外，还测试了各种生长基质，如珍珠岩、琼脂、香蕉皮、泥炭及其组合。分析重点在于微型植物的发芽能力、形态测量和生化分析。结果表明，在AS和NAS中使用的基于琼脂的可食用基质，可将发芽能力提高至95.00±0.30%，而泥炭在AS条件下的发芽能力仅为12.07±1.27%，在NAS条件下为6.07±0.35%。大多数生化分析表明，AS条件更适合罗勒微型植物的生产，与NAS条件相比，可提高干物质含量、总酚含量、总黄酮含量和总抗氧化能力。这些发现支持采用一种新的生态创新技术，该技术可提供有机罗勒微型植物，它们可与可食用琼脂基质一起完全使用。
