Yu Zhihui, Li Guangrong, Zheng Zhiqiang, Wang Hongjin, Yang Zujun
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
College of Life Sciences, Zaozhuang University, Zaozhuang 277100, China.
Plants (Basel). 2024 Aug 22;13(16):2333. doi: 10.3390/plants13162333.
The wild species (genome JJJJStSt) serves as a valuable germplasm resource providing novel diseases resistance and agronomically important genes for wheat improvement. Two wheat- partial amphiploids, TAI7045 (2n = 56) and 78784 (2n = 56), exhibit high resistance to stripe rust and powdery mildew, and their chromosome constitutions have been characterized. With the aim to transfer novel resistance genes from , the crosses of common wheat line MY11 with TAI7045 and 78784 were produced, and their individual F-F progenies were characterized using sequential non-denaturing fluorescence in situ hybridization (ND-FISH) and molecular markers. We identified a set of wheat- addition lines, involving the chromosomes 1St-J, 2St, 2St-J, 3St, 4J, 4St, 5St, 5J.St, 6J.J, and 7J. Above all, the stable wheat- small segmental translocation lines with chromosomes 4DS.4DL-4StL-4DL-4JL and 4DS.4DL-4StL-4DL were selected. Combining data from specific marker amplification and resistance evaluation, we mapped the gene(s) for resistance to powdery mildew and stripe rust in the 233.56-329.88 Mb region of the long arm of the 4St chromosome from the reference genome. The new wheat- introgressions will be used as novel germplasm for breeding purposes.
野生种（基因组JJJJStSt）是一种宝贵的种质资源，可为小麦改良提供新的抗病性和具有重要农艺价值的基因。两个小麦部分双体异源多倍体TAI7045（2n = 56）和78784（2n = 56）对条锈病和白粉病表现出高抗性，并且已对它们的染色体组成进行了鉴定。为了从小麦部分双体异源多倍体中转育新的抗性基因，构建了普通小麦品系MY11与TAI7045和78784的杂交组合，并使用连续非变性荧光原位杂交（ND-FISH）和分子标记对其F-F代个体进行了鉴定。我们鉴定出了一组小麦附加系，涉及1St-J、2St、2St-J、3St、4J、4St、5St、5J.St、6J.J和7J染色体。最重要的是，筛选出了具有4DS.4DL-4StL-4DL-4JL和4DS.4DL-4StL-4DL染色体的稳定小麦小片段易位系。结合特异性标记扩增数据和抗性评价，我们将抗白粉病和条锈病的基因定位到了参考基因组4St染色体长臂233.56 - 329.88 Mb区域。新的小麦渐渗系将作为新的种质用于育种目的。
