白色念珠菌中的肽转运

Peptide transport in Candida albicans.

作者信息

Logan D A, Becker J M, Naider F

出版信息

J Gen Microbiol. 1979 Sep;114(1):179-86. doi: 10.1099/00221287-114-1-179.

PMID:392049

Abstract

The tripeptide L-methionyl-L-methionyl-L-[METHYL-14C]methionine was taken up into Candida albicans by a saturable system with a pH optimum of 3.5, a temperature optimum of 37 degrees C and an apparent Km of 3.3 x 10(-5) M. Metabolic inhibitors such as sodium azide and dinitrophenol completely prevented uptake. Neither methionine nor dimethionine effectively competed with trimethionine uptake. (Leu)3, Gly-Met-Gly, acetyl-(Met)3, D-Met-L-Met-L-Met and Met-Met-Ile effectively competed with (Met)3 uptake, whereas (Lys)3, L-Met-L-Met-D-Met, D-Met-D-Met-D-Met, (Met)3 methyl ester and (Ala)3 did not. Trimethionine was rapidly hydrolysed by a peptidase after entry into the cell.

摘要

三肽L-甲硫氨酰-L-甲硫氨酰-L-[甲基-¹⁴C]甲硫氨酸通过一个饱和系统被白色念珠菌摄取，该系统的最适pH为3.5，最适温度为37℃，表观Km为3.3×10⁻⁵M。代谢抑制剂如叠氮化钠和二硝基苯酚完全阻止了摄取。甲硫氨酸和二甲硫氨酸都不能有效地与三甲硫氨酸的摄取竞争。(亮氨酸)₃、甘氨酰-甲硫氨酰-甘氨酸、乙酰-(甲硫氨酸)₃、D-甲硫氨酰-L-甲硫氨酰-L-甲硫氨酸和甲硫氨酰-甲硫氨酰-异亮氨酸能有效地与(甲硫氨酸)₃的摄取竞争，而(赖氨酸)₃、L-甲硫氨酰-L-甲硫氨酰-D-甲硫氨酸、D-甲硫氨酰-D-甲硫氨酰-D-甲硫氨酸、(甲硫氨酸)₃甲酯和(丙氨酸)₃则不能。三甲硫氨酸进入细胞后会被一种肽酶迅速水解。