Paolla Raimundo E Silva Joanilda, Silva Vasconcelos Priscilla Guimarães, Raimundo E Silva Joanda Paolla, Murata Ramiro Mendonça, Costa Edja Maria Melo de Brito
Postgraduate Program in Dentistry, State University of Paraiba, Campina Grande, Paraiba, Brazil.
Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraiba, João Pessoa, Paraiba, Brazil.
Nat Prod Res. 2024 Aug 29:1-5. doi: 10.1080/14786419.2024.2398214.
This work reports for the first time the evaluation of the cytotoxicity and inflammatory potential of extract treated with active charcoal in THP-1 monocytes. flower ethanolic extract was treated with 4% activated charcoal (TCEE). Later, THP-1 human monocyte cytotoxicity assay was performed using resazurin fluorometric method. Gene expression of inflammatory cytokines in THP-1 cells were evaluated through RT-PCR by ΔΔCt method using IL-1β, IL-6, IL-8, and TNF genes primers. Finally, antioxidant assay was carried out with DPPH radical scavenging method. TCEE had a LD of 592.5 µg/mL and did not induce pro-inflammatory gene expression in THP-1 cells after 6 h of treatment. Lastly, TCEE (AA% of 69.4 ± 1.4%) and CEE (AA% of 63.0 ± 0.9%) showed moderate antioxidant activity. treated flower extract showed low cytotoxicity in THP-1 monocytes and does not induce inflammation in THP-1 cells, in addition to presenting antioxidant potential.
这项工作首次报道了对经活性炭处理的花乙醇提取物在THP-1单核细胞中的细胞毒性和炎症潜力的评估。花乙醇提取物用4%的活性炭处理（TCEE）。随后，使用刃天青荧光法进行THP-1人单核细胞细胞毒性测定。通过RT-PCR，使用IL-1β、IL-6、IL-8和TNF基因引物，采用ΔΔCt法评估THP-1细胞中炎性细胞因子的基因表达。最后，用DPPH自由基清除法进行抗氧化测定。TCEE的半数致死剂量为592.5μg/mL，处理6小时后在THP-1细胞中未诱导促炎基因表达。最后，TCEE（抗氧化活性百分比为69.4±1.4%）和CEE（抗氧化活性百分比为63.0±0.9%）表现出中等抗氧化活性。经处理的花提取物在THP-1单核细胞中显示出低细胞毒性，在THP-1细胞中不诱导炎症，此外还具有抗氧化潜力。
