肠微生物群、免疫细胞与过敏性紫癜的因果关系：两步、两样本孟德尔随机化研究。
Causal relationships between gut microbiota, immune cell, and Henoch-Schönlein Purpura: a two-step, two-sample Mendelian randomization study.
机构信息
Department of Pediatric Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
出版信息
Front Immunol. 2024 Aug 14;15:1450544. doi: 10.3389/fimmu.2024.1450544. eCollection 2024.
BACKGROUND
Regulating the immune system is a crucial measure of gut microbiota (GM) that influences the development of diseases. The causal role of GM on Henoch-Schönlein Purpura (HSP) and whether it can be mediated by immune cells is still unknown.
METHODS
We performed a two-sample Mendelian randomization study using an inverse variance weighted (IVW) method to examine the causal role of GM on HSP and the mediation effect of immune cells between the association of GM and HSP.
RESULTS
We demonstrated the causal relationships between 14 axas and 6 pathways with HSP. Additionally, we identified 9 immune cell characteristics associated with HSP. Importantly, through mediation MR analysis, we identified several immune cell characteristics that mediate the impact of GM on HSP. For instance, Genus_Blautia affects HSP via Monocyte (HLA DR on CD14+ CD16- monocyte) and Monocyte (HLA DR on monocyte). The proportion of mediation effects further elucidated the complex dynamics between GM exposure, immune markers, and their combined impact on HSP.
CONCLUSION
The study suggested a causal relationship between GM and HSP, which may be mediated by immune cells.
背景
调节免疫系统是影响疾病发展的肠道微生物群（GM）的关键措施。GM 对过敏性紫癜（HSP）的因果作用，以及它是否可以通过免疫细胞介导，目前尚不清楚。
方法
我们使用逆方差加权（IVW）方法进行了两样本孟德尔随机化研究，以检验 GM 对 HSP 的因果作用以及 GM 与 HSP 之间关联的免疫细胞的中介效应。
结果
我们证明了 14 个轴和 6 条通路与 HSP 之间存在因果关系。此外，我们确定了 9 种与 HSP 相关的免疫细胞特征。重要的是，通过中介 MR 分析，我们确定了几种免疫细胞特征可以介导 GM 对 HSP 的影响。例如，属 Blautia 通过单核细胞（CD14+CD16-单核细胞上的 HLA DR）和单核细胞（单核细胞上的 HLA DR）影响 HSP。中介效应的比例进一步阐明了 GM 暴露、免疫标志物及其对 HSP 的综合影响之间的复杂动态。
结论
该研究表明 GM 与 HSP 之间存在因果关系，其可能通过免疫细胞介导。
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