Chen Jiabin, Yu Xuzhou, Wu XiaoYu, Chai Kequn, Wang Sheng
The Second Clinical Medical College, Zhejiang Chinese Medicine University, Hangzhou, Zhejiang 310012, China.
Department of Oncology, Tongde Hospital of Zhejiang, Hangzhou, Zhejiang 310012, China.
J Cancer. 2024 Feb 4;15(7):1890-1897. doi: 10.7150/jca.92699. eCollection 2024.
Regulating the immune system is a crucial measure of gut microbiota (GM) that influences the development of diseases. The causal role of GM on Non-small cell lung cancer (NSCLC) and whether it can be mediated by immune cells is still unknown. We performed a two-step, two-sample Mendelian randomization study with an Inverse variance weighted (IVW) approach to investigate the causal role of GM on NSCLC and the mediation effect of immune cells between the association of GM and NSCLC. MR analyses determined the protective effects of 6 genera on NSCLC (Bacteroides, Roseburia, Alistipes, Methanobrevibacter, Ruminococcus gauvreauii group, and Peptococcus). In addition, 38 immune cell traits were suggestively associated with NSCLC. Of note, the mediation MR illustrated the causal role of Genus-Peptococcus on NSCLC (Total effect IVW: OR = 0.790, 95% CI [0.657, 0.950], P = 0.012) was to a large proportion mediated by CD45 on HLA DR CD4 in TBNK panel (-034 (95% CI [-0.070, -0.005]; P = 0.037), accounting for 14.4% of Total effect). The study suggested a causal relationship between GM and NSCLC, which may be mediated by immune cells.
调节免疫系统是肠道微生物群（GM）的一项关键措施，它会影响疾病的发展。GM对非小细胞肺癌（NSCLC）的因果作用以及它是否能由免疫细胞介导仍不清楚。我们采用逆方差加权（IVW）方法进行了一项两步、两样本孟德尔随机化研究，以调查GM对NSCLC的因果作用以及免疫细胞在GM与NSCLC关联之间的中介作用。孟德尔随机化分析确定了6个属对NSCLC的保护作用（拟杆菌属、罗斯氏菌属、艾氏菌属、甲烷短杆菌属、戈氏瘤胃球菌群和消化球菌属）。此外，38种免疫细胞特征与NSCLC存在提示性关联。值得注意的是，中介孟德尔随机化分析表明，消化球菌属对NSCLC的因果作用（IVW总效应：OR = 0.790，95% CI [0.657, 0.950]，P = 0.012）在很大程度上是由TBNK细胞亚群中CD45对HLA DR CD4的作用介导的（-0.34（95% CI [-0.070, -0.005]；P = 0.037），占总效应的14.4%）。该研究表明GM与NSCLC之间存在因果关系，这可能由免疫细胞介导。
