Grundmann E, Vollmer E
Pathol Res Pract. 1985 Jan;179(3):304-9. doi: 10.1016/S0344-0338(85)80139-4.
2 X 10(6) ascites tumor cells of a DMBA-induced rat mammary adenocarcinoma (HH9-cl 14) were injected subcutaneously into tumor-free syngeneic female rats. Investigations were made, in addition to conventional paraffin histology and light microscopy, with immunohistological methods using poly-and monoclonal antisera. From 10 minutes to 2 hrs after inoculation carcinoma cells were seen inert either as single cells or as little clusters between connective and fatty tissue. After 6 hrs the number of tumor cells had decreased, and an increasing quantity of macrophages were found in close contact with them. At this stage some lymphocytes, too, appeared in the inoculation area. 10 minutes after injection, tumor cells were already entering regional lymph nodes and could be observed in the subcapsular sinuses. 2 hours later they had almost traversed the intermediate and medullary sinuses. Up to 6 hours after injection, increasing numbers of macrophages and sinus endothelial cells were seen, and the lymph node's appearance manifested hyperplasia of the paracortical zone. 4 and 6 hrs after injection, the total number of tumor cells in regional nodes decreased again noticeably, and sometimes tumor cells had disappeared. The first lymph node metastases appeared 28 days after inoculation.
将2×10(6)个由二甲基苯蒽（DMBA）诱导的大鼠乳腺腺癌（HH9-cl 14）腹水肿瘤细胞皮下注射到无肿瘤的同基因雌性大鼠体内。除了常规石蜡组织学和光学显微镜检查外，还使用多克隆和单克隆抗血清通过免疫组织学方法进行研究。接种后10分钟至2小时，癌细胞呈惰性，以单个细胞或结缔组织与脂肪组织之间的小簇形式存在。6小时后，肿瘤细胞数量减少，发现越来越多的巨噬细胞与它们紧密接触。在此阶段，接种区域也出现了一些淋巴细胞。注射后10分钟，肿瘤细胞已进入局部淋巴结，并可在被膜下窦中观察到。2小时后，它们几乎穿过了中间窦和髓窦。注射后长达6小时，可见越来越多的巨噬细胞和窦内皮细胞，淋巴结外观表现为副皮质区增生。注射后4小时和6小时，局部淋巴结中肿瘤细胞总数再次明显减少，有时肿瘤细胞已消失。首次淋巴结转移出现在接种后28天。
