Department of MCD Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA.
Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, California 95064, USA.
RNA. 2024 Oct 16;30(11):1513-1528. doi: 10.1261/rna.080103.124.
is an important model organism for human health and disease, with foundational contributions to the understanding of gene expression and tissue patterning in animals. An invaluable tool in modern gene expression research is the presence of a high-resolution ribosome structure, though no such structure exists for Here, we present a high-resolution single-particle cryogenic electron microscopy (cryo-EM) reconstruction and molecular model of a ribosome, revealing a significantly streamlined animal ribosome. Many facets of ribosome structure are conserved in , including overall ribosomal architecture and the mechanism of cycloheximide, whereas other facets, such as expansion segments and eL28, are rapidly evolving. We identify uL5 and uL23 as two instances of tissue-specific ribosomal protein paralog expression conserved in , suggesting that ribosomes vary across tissues. The ribosome structure will provide a basis for future structural, biochemical, and genetic studies of translation in this important animal system.
是人类健康和疾病的重要模式生物，对动物基因表达和组织模式的理解做出了基础性贡献。高分辨率核糖体结构是现代基因表达研究中非常宝贵的工具，但 中不存在这样的结构。在这里，我们展示了一个高分辨率的单颗粒冷冻电子显微镜（cryo-EM）重构和分子模型，揭示了一个明显精简的动物核糖体。核糖体结构的许多方面在 中都得到了保守，包括核糖体的整体结构和环己酰亚胺的作用机制，而其他方面，如扩展片段和 eL28，则在快速进化。我们确定 uL5 和 uL23 是在 中保守的组织特异性核糖体蛋白同工型表达的两个实例，表明 核糖体在不同组织中存在差异。该 核糖体结构将为未来在这个重要的动物系统中进行翻译的结构、生化和遗传研究提供基础。
