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与人类免疫球蛋白重链基因增强子结合的核因子。

Nuclear factors binding to the human immunoglobulin heavy-chain gene enhancer.

作者信息

Maeda H, Araki K, Kitamura D, Wang J, Watanabe T

出版信息

Nucleic Acids Res. 1987 Apr 10;15(7):2851-69. doi: 10.1093/nar/15.7.2851.

DOI:10.1093/nar/15.7.2851
PMID:3031612
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340703/
Abstract

The human immunoglobulin heavy chain (IgH) gene contains at least two tissue-specific regulatory regions, which are similar to the mouse IgH gene. One is the J-C enhancer and another is located in the 5' promoter region. Using an electrophoretic mobility shift assay and DNase I footprint, we have examined the interaction of factors in B cell nuclear extracts with the two regulatory regions of the human IgH gene. We have identified a nuclear factor in mouse B cell nuclear extracts which bound to specific sequence in the human IgH enhancer. This factor is apparently not present in mouse fibroblast nuclear extracts. We also found factor(s) which bound to the highly conserved octanucleotide sequence within the human IgH enhancer and 5' promoter regions.

摘要

人类免疫球蛋白重链（IgH）基因包含至少两个组织特异性调控区，这与小鼠IgH基因相似。一个是J-C增强子，另一个位于5'启动子区域。利用电泳迁移率变动分析和DNase I足迹法，我们检测了B细胞核提取物中的因子与人类IgH基因的两个调控区之间的相互作用。我们在小鼠B细胞核提取物中鉴定出一种与人类IgH增强子中的特定序列结合的核因子。该因子在小鼠成纤维细胞核提取物中显然不存在。我们还发现了与人类IgH增强子和5'启动子区域内高度保守的八聚体序列结合的因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/1c3d041088cf/nar00251-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/099db0a4a1a8/nar00251-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/e5f45d6240ca/nar00251-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/e4adf90efa11/nar00251-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/3c26faaf4524/nar00251-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/26900cac9359/nar00251-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/7e2dad687ed9/nar00251-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/1c3d041088cf/nar00251-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/099db0a4a1a8/nar00251-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/e5f45d6240ca/nar00251-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/e4adf90efa11/nar00251-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/3c26faaf4524/nar00251-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/26900cac9359/nar00251-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/7e2dad687ed9/nar00251-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd5/340703/1c3d041088cf/nar00251-0087-a.jpg

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本文引用的文献

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Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.转染至淋巴细胞中的免疫球蛋白重链基因的表达与调控
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Multiple negative elements in a gene that codes for an extracellular matrix protein, collagen X, restrict expression to hypertrophic chondrocytes.
编码细胞外基质蛋白（胶原蛋白X）的基因中的多个负调控元件将表达限制在肥大软骨细胞中。
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Pi, a pre-B-cell-specific enhancer element in the immunoglobulin heavy-chain enhancer.Pi，免疫球蛋白重链增强子中一个前B细胞特异性增强子元件。
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Mol Cell Biol. 1988 Oct;8(10):3975-87. doi: 10.1128/mcb.8.10.3975-3987.1988.
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Nucleic Acids Res. 1988 Jul 11;16(13):6085-96. doi: 10.1093/nar/16.13.6085.
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Nucleic Acids Res. 1988 Jun 10;16(11):4967-88. doi: 10.1093/nar/16.11.4967.
用环己酰亚胺处理诱导小鼠成纤维细胞中免疫球蛋白基因表达。
J Exp Med. 1984 Dec 1;160(6):1937-42. doi: 10.1084/jem.160.6.1937.
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Two regulatory elements for immunoglobulin kappa light chain gene expression.免疫球蛋白κ轻链基因表达的两个调控元件。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7041-5. doi: 10.1073/pnas.81.22.7041.
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Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4. doi: 10.1073/pnas.81.9.2650.
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Activation of a translocated human c-myc gene by an enhancer in the immunoglobulin heavy-chain locus.免疫球蛋白重链基因座中的增强子对易位的人类c-myc基因的激活作用。
Nature. 1984;307(5949):334-40. doi: 10.1038/307334a0.
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DNase I hypersensitive sites in the chromatin of human mu immunoglobulin heavy-chain genes.人类μ免疫球蛋白重链基因染色质中的脱氧核糖核酸酶I超敏位点
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Transcription enhancer identified near the human C mu immunoglobulin heavy chain gene is unavailable to the translocated c-myc gene in a Burkitt lymphoma.在人类Cμ免疫球蛋白重链基因附近鉴定出的转录增强子在伯基特淋巴瘤中对易位的c-myc基因不可用。
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A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.一种淋巴细胞特异性细胞增强子位于免疫球蛋白重链基因连接区的下游。
Cell. 1983 Jul;33(3):729-40. doi: 10.1016/0092-8674(83)90015-6.
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A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.一种组织特异性转录增强子元件位于重排的免疫球蛋白重链基因的主要内含子中。
Cell. 1983 Jul;33(3):717-28. doi: 10.1016/0092-8674(83)90014-4.