Bukholm G, Degré M
J Interferon Res. 1985 Winter;5(1):45-53. doi: 10.1089/jir.1985.5.45.
The effect of human gamma interferon (HuIFN-gamma) on the invasiveness of Salmonella typhimurium, Shigella flexneri and Yersinia enterocolitica serotype 0:3 in HEp-2 cell cultures was examined. The intracellular and extracellular bacteria were identified by a combination of Nomarski differential interference contrast microscopy and UV incident light microscopy applied on the same microscope. Pretreatment of HEp-2 cells with HuIFN-gamma reduced in a dose dependent manner the number of S. typhimurium bacteria per cell and the proportion of cells containing bacteria. Maximum inhibitory effect was observed with approximately 10 units per ml of HuIFN-gamma. The inhibitory effect of interferon as well as the antiviral effect was eliminated when the preparation was neutralized with monoclonal anti-interferon globulin and was acidified to pH 2. Murine gamma interferon did not influence invasiveness of S. typhimurium. No effect of interferon was observed when the bacteria were incubated in cell cultures at 4 degrees C. Invasiveness of S. flexneri and Y. enterocolitica was not influenced by treatment of cells with HuIFN.
研究了人γ干扰素（HuIFN-γ）对鼠伤寒沙门氏菌、福氏志贺氏菌和小肠结肠炎耶尔森氏菌0:3血清型在HEp-2细胞培养物中侵袭性的影响。通过在同一显微镜上应用诺马斯基微分干涉对比显微镜和紫外入射光显微镜相结合的方法来鉴定细胞内和细胞外细菌。用HuIFN-γ预处理HEp-2细胞以剂量依赖方式减少了每个细胞中鼠伤寒沙门氏菌的数量以及含有细菌的细胞比例。在约每毫升10单位的HuIFN-γ时观察到最大抑制作用。当制剂用单克隆抗干扰素球蛋白中和并酸化至pH 2时，干扰素的抑制作用以及抗病毒作用均被消除。鼠γ干扰素不影响鼠伤寒沙门氏菌的侵袭性。当细菌在4℃的细胞培养物中孵育时，未观察到干扰素的作用。用HuIFN处理细胞对福氏志贺氏菌和小肠结肠炎耶尔森氏菌的侵袭性没有影响。
