Bukholm G, Berdal B P, Haug C, Degré M
Infect Immun. 1984 Jul;45(1):62-6. doi: 10.1128/iai.45.1.62-66.1984.
The effect of mouse fibroblast interferon on Salmonella typhimurium infection in infant mice was examined. The lethality to mice that had been given S. typhimurium intragastrically was significantly reduced in a dose-dependent manner when the mice were pretreated with fibroblast interferon. Lower doses of interferon delayed the development of disease. Interferon neutralized with anti-interferon globulin did not influence the lethality of S. typhimurium to mice. In mice treated with interferon there was also a reduced invasiveness of S. typhimurium in intestinal epithelial cells in vivo. It was further demonstrated in an in vitro system that interferon pretreatment of mouse L-929 cells inhibited the invasiveness of the bacteria in a dose-dependent manner. The in vitro inhibition was neutralized with anti-interferon globulin. The results indicate that interferon inhibits Salmonella bacteria from invading cells and establishing an intracellular state of infection. This may represent an important factor in the pathogenesis of disease.
研究了小鼠成纤维细胞干扰素对幼鼠鼠伤寒沙门氏菌感染的影响。当用成纤维细胞干扰素对小鼠进行预处理时，经胃内给予鼠伤寒沙门氏菌的小鼠的致死率以剂量依赖的方式显著降低。较低剂量的干扰素延缓了疾病的发展。用抗干扰素球蛋白中和的干扰素对鼠伤寒沙门氏菌对小鼠的致死率没有影响。在用干扰素治疗的小鼠中，鼠伤寒沙门氏菌在体内肠上皮细胞中的侵袭性也降低。在体外系统中进一步证明，对小鼠L-929细胞进行干扰素预处理以剂量依赖的方式抑制了细菌的侵袭性。体外抑制作用被抗干扰素球蛋白中和。结果表明，干扰素抑制沙门氏菌侵入细胞并建立细胞内感染状态。这可能是疾病发病机制中的一个重要因素。
