School of Agriculture Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Research Laboratory for Analytical Instrument and Electrochemistry Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Food Chem. 2025 Jan 1;462:140988. doi: 10.1016/j.foodchem.2024.140988. Epub 2024 Aug 24.
The 3,3',5,5'-tetramethylbenzidine-HO (TMB-HO) platform has gained widespread use for rapid detection of various analytes in foods. However, the existing TMB-HO platforms suffer from limited accuracy, as their signal output is confined to the visible region, which is prone to interference from various food colorants in real samples. To address this challenge, a novel Au@Os-mediated TMB-HO platform is developed for both rapid and accurate detection of analytes in foods. The prepared Au@Os NPs exhibit remarkable peroxidase-like activity, making the platform display dual absorption peaks in visible and near-infrared (NIR) regions, respectively. This Au@Os-mediated TMB-HO platform exhibited three linear ranges across different concentrations of ziram from 1-100, 150-600, and 800-2000 nM with limit of detection (LOD) 7.9 nM and limit of quantification (LOQ) 24.15 nM respectively. Further, the Au@Os-mediated TMB-HO platform was also used for rapid and accurate detection of ziram in real food samples like apple, tomato, and black tea.
3,3',5,5'-四甲基联苯胺-HO（TMB-HO）平台因其能够快速检测各种食品中的分析物而得到了广泛的应用。然而，现有的 TMB-HO 平台的准确性有限，因为它们的信号输出仅限于可见区域，这容易受到实际样品中各种食品着色剂的干扰。为了解决这一挑战，开发了一种新型的 Au@Os 介导的 TMB-HO 平台，用于快速准确地检测食品中的分析物。制备的 Au@Os NPs 表现出显著的过氧化物酶样活性，使平台在可见和近红外（NIR）区域分别显示出两个吸收峰。该 Au@Os 介导的 TMB-HO 平台在不同浓度的锌尘范围内表现出三个线性范围，从 1-100、150-600 和 800-2000 nM，检测限（LOD）为 7.9 nM，定量限（LOQ）为 24.15 nM。此外，该 Au@Os 介导的 TMB-HO 平台还用于快速准确地检测苹果、番茄和红茶等实际食品样品中的锌尘。
