Department of Oral and Maxillofacial Surgery, Dental School, University of Ghana/Oral and Maxillofacial Surgery Unit, Korle-Bu Teaching Hospital, Accra, Ghana.
Department of Oral and Maxillofacial Surgery, Dental School, University of Ghana/Oral and Maxillofacial Surgery Unit, Korle-Bu Teaching Hospital, Accra, Ghana.
Clinics (Sao Paulo). 2024 Aug 31;79:100477. doi: 10.1016/j.clinsp.2024.100477. eCollection 2024.
To determine the prevalence and association of HPV and Herpesviruses in saliva and tissue samples of patients with orofacial tumors.
Biopsies of tumors were done, and saliva samples were collected from patients with orofacial tumors for the determination of viruses using nested multiplex PCR. Independent variables were sex, age, comorbidities, tumor stage, and length of stay. Outcome variables were the presence or absence of herpesviruses and HPV. Descriptive summaries and inferential statistics were done.
A hundred patients were included in the study. Prevalence of herpesviruses and HPV were 17.6 % and 57.0 % in tumors, and 48.3 % and 60.0 % in the saliva of patients respectively. Herpesviruses detected included EBV (21.3 %), HHV-7 (11.2 %), CMV (6.7 %), HSV-1 (5.1 %), HSV-2 (1.1 %), VZV (1.1 %), and Kaposi sarcoma virus (0.6 %). The most prevalent HPV genotypes were HPV-42 (29 %), HPV-43 (22.7 %), HPV-52 (22.2 %), HPV-39 (18.8 %), and HPV-18 (9.1 %). The odds of EBV being detected in malignant orofacial tumors were 2 times that of benign orofacial tumors. HPV DNA in the saliva of patients with orofacial tumors was 69.7 %, compared to 18.2 % of the control sample (p < 0.001). The median length of stay for all participants was 6.5 days, those associated with viruses stayed longer.
There was a high prevalence of Herpesviruses and HPV in saliva and tumor samples of patients with orofacial tumors, signalling some potential for more work to be done in this area.
确定口腔颌面部肿瘤患者唾液和组织样本中 HPV 和疱疹病毒的流行率和相关性。
对肿瘤进行活检，并从口腔颌面部肿瘤患者中采集唾液样本，使用巢式多重 PCR 检测病毒。自变量为性别、年龄、合并症、肿瘤分期和住院时间。观察变量为疱疹病毒和 HPV 的存在或缺失。进行描述性总结和推断性统计。
本研究共纳入 100 例患者。肿瘤中疱疹病毒和 HPV 的患病率分别为 17.6%和 57.0%，患者唾液中分别为 48.3%和 60.0%。检测到的疱疹病毒包括 EBV（21.3%）、HHV-7（11.2%）、CMV（6.7%）、HSV-1（5.1%）、HSV-2（1.1%）、VZV（1.1%）和卡波西肉瘤病毒（0.6%）。最常见的 HPV 基因型是 HPV-42（29%）、HPV-43（22.7%）、HPV-52（22.2%）、HPV-39（18.8%）和 HPV-18（9.1%）。在恶性口腔颌面部肿瘤中检测到 EBV 的几率是良性口腔颌面部肿瘤的 2 倍。口腔颌面部肿瘤患者唾液中的 HPV DNA 为 69.7%，而对照组为 18.2%（p<0.001）。所有参与者的中位住院时间为 6.5 天，与病毒相关的患者住院时间更长。
口腔颌面部肿瘤患者唾液和肿瘤样本中疱疹病毒和 HPV 的流行率较高，这表明在该领域还需要进一步研究。
