Eckhardt L A, Birshtein B K
Mol Cell Biol. 1985 Apr;5(4):856-68. doi: 10.1128/mcb.5.4.856-868.1985.
Five gamma 2a-producing cell lines, all derived from the gamma 2b-producing mouse myeloma MPC11, were analyzed for changes in gene structure. Also examined was a cell line (ICR9.7.1) that acts as an intermediate to some of these class switches. All six of the MPC11 variants have undergone DNA rearrangement. Rearrangement sites in the gamma 2a-producing cells are different in each case and generally do not involve tandem repeat sequences. An enhancer for heavy chain gene transcription was deleted in at least one of these cell lines as a result of its class-switch rearrangement. DNA sequence analysis of cloned genes from two of the MPC11 variants revealed shared sequences at their rearrangement breakpoints. The same sequences are found at the breakpoints of two additional immunoglobulin gene rearrangements in MPC11.
对五个均源自产生γ2b的小鼠骨髓瘤MPC11的产生γ2a的细胞系进行了基因结构变化分析。还检测了一个细胞系（ICR9.7.1），它是其中一些类别转换的中间类型。所有六个MPC11变体都经历了DNA重排。每个产生γ2a的细胞中的重排位点都不同，并且一般不涉及串联重复序列。由于类别转换重排，这些细胞系中至少有一个缺失了重链基因转录增强子。对两个MPC11变体的克隆基因进行的DNA序列分析揭示了它们重排断点处的共享序列。在MPC11的另外两个免疫球蛋白基因重排的断点处也发现了相同的序列。
